caa a22 4500 477089763 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254958 doi (NATIONALLICENCE)springer-10.1007/BF02254958 Differential immunostaining of plant chromosomes by antibodies recognizing acetylated histone H4 variants [Elektronische Daten] [Andreas Houben, Nikolai Belyaev, Bryan Turner, Ingo Schubert] Metaphase chromosomes ofVicia faba were exposed to antibodies recognizing defined acetylated isoforms of histone H4. After indirect immunostaining with antibodies directed against H4 acetylated on lysines 5, 8 and 12 respectively, the entire chromosome complement was labelled. The brightest signal appeared at the nucleolus organizing region (NOR). The large genetically inert heterochromatic regions, which are composed of late replicating tandemly repetitive DNA sequences, remained unlabelled. Thus, the chromosomal distribution of histones H4 acetylated at positions of lysine 5, 8 and 12 is broadly correlated with the intensity of transcription and the sequence of replication of the field bean chromatin during interphase. Antibodies against H4 acetylated at lysine 16 also caused a strong signal at the NOR but otherwise a uniform fluorescence along the chromosome. Rapid Science Publishers, 1996 acetylated histone H4 nationallicence heterochromatin nationallicence indirect immunofluorescence nationallicence nucleolus organizing region nationallicence Vicia faba nationallicence Houben Andreas Institut für Pflanzengenetick und Kulturpflanzenforschung, D 06466, Gatersleben, Germany aut Belyaev Nikolai Chromatin and Gene Expression Group, Department of Anatomy, Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut Turner Bryan Chromatin and Gene Expression Group, Department of Anatomy, Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut Schubert Ingo Institut für Pflanzengenetick und Kulturpflanzenforschung, D 06466, Gatersleben, Germany aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 191-194 0967-3849 4:3<191 1996 4 10577 https://doi.org/10.1007/BF02254958 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254958 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Houben Andreas Institut für Pflanzengenetick und Kulturpflanzenforschung, D 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Belyaev Nikolai Chromatin and Gene Expression Group, Department of Anatomy, Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Turner Bryan Chromatin and Gene Expression Group, Department of Anatomy, Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Schubert Ingo Institut für Pflanzengenetick und Kulturpflanzenforschung, D 06466, Gatersleben, Germany aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 191-194 0967-3849 4:3<191 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer