caa a22 4500 477089852 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02263674 doi (NATIONALLICENCE)springer-10.1007/BF02263674 Fluorescence lifetime imaging: an emerging technique in fluorescence microscopy [Elektronische Daten] [C. Morgan, A. Mitchell] Fluorescence microscopy is an important tool for biological research, in part because of the extremely high detection sensitivity that can be achieved, but also because fluorescent molecules can be used as probes on account of their environmental responsiveness, for example to measure intracellular pH or metal ion concentration. Unfortunately, the environmental sensitivity can sometimes be a source of problems because of enhancement of ‘quenching', which can make it very difficult to relate emission intensity to the amount of fluorophore present. The measured intensity is essentially proportional to the product of the amount of fluorophore present in the sample and the local quantum yield of the fluorophore (the quantum yield can be thought of as the probability that an excited molecule decays by fluorescence emission rather than by other non-radiative processes). This is a particular difficulty in an environment such as a cell or tissue slice in which quantum yield and fluorophore concentration can both vary within the sample. Ideally we would wish to be able to measure the quantum yield of fluorescence as well as the fluorescence intensity, as this would allow environmental effects to be compensated for. Unfortunately, this is not at all easy, and indirect means to achieve the same goal are more appropriate. A recently introduced technique,fluorescence lifetime imaging (Morganet al. 1992, Wanget al. 1992), offers one such means to improve quantification of fluorescence microscopy. In addition, as will be explained, the technique offers the prospect of significantly improving detection sensitivity in appropriate circumstances. Rapid Science Publishers, 1996 Morgan C. Department of Biological Sciences, Science Research Institute, University of Salford, M5 4WT, Salford, UK aut Mitchell A. aut Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 261-263 0967-3849 4:4<261 1996 4 10577 https://doi.org/10.1007/BF02263674 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02263674 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morgan C. Department of Biological Sciences, Science Research Institute, University of Salford, M5 4WT, Salford, UK aut NATIONALLICENCE 700 1- Mitchell A. aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 261-263 0967-3849 4:4<261 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer