caa a22 4500 477089860 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02263683 doi (NATIONALLICENCE)springer-10.1007/BF02263683 Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22-p21 tumour-suppressor region by two-colour fluorescence in situ hybridization [Elektronische Daten] [Anna Szeles, Svetlana Bajalica-Lagercrantz, Annika Lindblom, Tamara Lushnikova, Vladimir Kashuba, Stephan Imreh, Magnus Nordenskjöld, George Klein, R. Zabarovsky] ANotl-linking clone NL1-210 (D3S1656) that contains the human MAP kinase activated protein kinase (3PK) gene was localized to 3p21.2 on DAPI-banded and propidium iodide (R-bands)-stained chromosomes by fluorescencein situ hybridization (FISH). For more precise localization of 3PK, two cosmid probes were used as a frame. In order to establish this frame, twoNotl-linking clones, NL2-008 (D3S1648) and NL3-003 (D3S3872) were used to screen the cosmid library for locus extension. They mapped to 3p21 and were found to belong to two separate contigs ofNotl-jumping and linking clones. Using FISH on DAPI-banded metaphase chromosomes, we have determined the precise localization of cosNL2-008 and cosNL3-003 to 3p21.2-p21.1 and 3p22-p21.3 respectively. The 3PK gene was localized to the 3p21.2 region within this frame by two-colour FISH. The orientation of the probes are tel-D3S3872-3PK-D3S1648-cen. Rapid Science Publishers, 1996 FISH nationallicence human chromosome 3 nationallicence mapping nationallicence 3PK D351656 nationallicence Szeles Anna Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut Bajalica-Lagercrantz Svetlana Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut Lindblom Annika Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut Lushnikova Tamara Laboratory of Molecular Genetics, Institute of Chemical Physics and Biophysics, 23 EE0026, Tallinn, Estonia aut Kashuba Vladimir Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut Imreh Stephan Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut Nordenskjöld Magnus Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut Klein George Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut Zabarovsky R. Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 310-313 0967-3849 4:4<310 1996 4 10577 https://doi.org/10.1007/BF02263683 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02263683 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szeles Anna Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut NATIONALLICENCE 700 1- Bajalica-Lagercrantz Svetlana Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut NATIONALLICENCE 700 1- Lindblom Annika Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut NATIONALLICENCE 700 1- Lushnikova Tamara Laboratory of Molecular Genetics, Institute of Chemical Physics and Biophysics, 23 EE0026, Tallinn, Estonia aut NATIONALLICENCE 700 1- Kashuba Vladimir Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut NATIONALLICENCE 700 1- Imreh Stephan Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut NATIONALLICENCE 700 1- Nordenskjöld Magnus Department of Clinical Genetics, Karolinska Hospital, S-17176, Stockholm, Sweden aut NATIONALLICENCE 700 1- Klein George Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut NATIONALLICENCE 700 1- Zabarovsky R. Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, PO Box 280, S-17177, Stockholm, Sweden aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 310-313 0967-3849 4:4<310 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer