caa a22 4500 477089895 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02263680 doi (NATIONALLICENCE)springer-10.1007/BF02263680 The human/mouse imprinted genes IGF2, H19, SNRPN and ZNF127 map to two conserved autosomal clusters in a marsupial [Elektronische Daten] [R. Toder, S. Wilcox, M. Smithwick, J. Graves] The four genesIGF2, H19, SNRPN andZNF127 are imprinted in mouse and human.IGF2 andH19 form one conserved cluster on the distal part of mouse chromosome 7 and human chromosome 11p15.5, whereasSNRPN andZNF127 form another on the middle of mouse chromosome 7 and on human chromosome 15q11-13. We have explored the evolution of these imprinted regions by cloning and mappingIGF2, H19, SNRPN andZNF127 homeologues in marsupials. Specifically, we wished to determine whether the arrangements were shared in eutherian and marsupial mammals, and to determine whether they lay on autosomes, or on the X, as might be predicted by the hypothesis that imprinting evolved from X inactivation. Using fluorescencein situ hybridization, we localized the marsupial homeologues ofIGF2 andH19 to the distal part of tammar wallaby chromosome 2p and the marsupial homeologues ofSNRPN andZNF127 to the middle of chromosome 1q. Thus, these genes were originally organized in two separate autosomal clusters in the therian ancestor 180 million years ago, the conservation of which may suggest a functional relationship. The autosomal location of these clusters does not suggest a recent evolutionary relationship between imprinting and X chromosome inactivation. Rapid Science Publishers, 1996 chromosome in situ suppression hybridization nationallicence functional clusters nationallicence gene mapping nationallicence genomic imprinting nationallicence Toder R. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut Wilcox S. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut Smithwick M. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut Graves J. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 295-300 0967-3849 4:4<295 1996 4 10577 https://doi.org/10.1007/BF02263680 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02263680 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toder R. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut NATIONALLICENCE 700 1- Wilcox S. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut NATIONALLICENCE 700 1- Smithwick M. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut NATIONALLICENCE 700 1- Graves J. School of Genetics and Human Variation, La Trobe University, 3083, Melbourne, Australia aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 295-300 0967-3849 4:4<295 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer