caa a22 4500 477089925 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02263678 doi (NATIONALLICENCE)springer-10.1007/BF02263678 Distribution and linkage of repetitive clusters from the heterochromatic region of human chromosome 22 [Elektronische Daten] [Roman Müllenbach, Carsten Pusch, Karlheinz Holzmann, Ron Suijkerbuijk, Nikolaus Blin] The pericentric regions of eukaryotic chromosomes consist of several types of repetitive DNA families. In human chromosome 22, the organization of such families was studied in more detail. In addition to the known families of alpha and beta repeats, an additional repeat with a 48-bp motif was previously assigned to 22pter-q11. Here, we report in more detail the distribution of these repeat families, applying pulsed-field gel electrophoresis, fluorescencein situ hybridization and physical linkage on cosmid recombinants. At least two clusters of 48-bp repeats are localized on chromosome 22, one on the distal p-arm and one in the region 22cen-q11. Cosmids from a chromosome 22 library, containing both 48-bp and β-repeats, link both arrays on 22p and define their maximum distances to less than 44 kb. Loss of 48-bp repeat sequences in a Di-George cell line carrying a deletion in 22q11 suggests the presence of a second cluster in 22q11, a distribution supported by (fluorescenein situ hybridization)-FISH signal analysis. As additional members of the 48-bp repeat family can be found on all acrocentric chromosomes it remains to be determined whether the distribution seen on chromosome 22 is also common in other human acrocentric chromosomes. Rapid Science Publishers, 1996 centromere nationallicence cosmid clone nationallicence 48-bp motif nationallicence fluorescence in situ hybridization nationallicence satellite DNA nationallicence Müllenbach Roman ICRF Molecular Oncology Unit, Institute of Child Health, 30 Guildford Street, London, UK aut Pusch Carsten Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstrasse 27, D-72074, Tübingen, Germany aut Holzmann Karlheinz Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstrasse 27, D-72074, Tübingen, Germany aut Suijkerbuijk Ron Cancer Center, Southwest Biomedical Research Institute, Scottsdale, AZ, USA aut Blin Nikolaus Department of Human Genetics, University Hospital, Nijmegen, The Netherlands aut Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 282-287 0967-3849 4:4<282 1996 4 10577 https://doi.org/10.1007/BF02263678 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02263678 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müllenbach Roman ICRF Molecular Oncology Unit, Institute of Child Health, 30 Guildford Street, London, UK aut NATIONALLICENCE 700 1- Pusch Carsten Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstrasse 27, D-72074, Tübingen, Germany aut NATIONALLICENCE 700 1- Holzmann Karlheinz Institute of Anthropology & Human Genetics, University of Tübingen, Wilhelmstrasse 27, D-72074, Tübingen, Germany aut NATIONALLICENCE 700 1- Suijkerbuijk Ron Cancer Center, Southwest Biomedical Research Institute, Scottsdale, AZ, USA aut NATIONALLICENCE 700 1- Blin Nikolaus Department of Human Genetics, University Hospital, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/4(1996-06-01), 282-287 0967-3849 4:4<282 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer