caa a22 4500 47708995X CHVBK 20180405111513.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02261721 doi (NATIONALLICENCE)springer-10.1007/BF02261721 Characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic DNA on two Triticum aestivum—Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus [Elektronische Daten] [Richard Wang, Xue-Yong Zhang] Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination. Rapid Science Publishers, 1996 in situ hybridization nationallicence intermediate wheatgrass nationallicence translocation nationallicence virus resistance nationallicence wheat nationallicence Wang Richard USDA-ARS (United States Department of Agriculture-Agricultural Research Service) Forage and Range Research Laboratory, Utah State University, 84322-6300, Logan, UT, USA aut Zhang Xue-Yong Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081, Beijing, China aut Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 583-587 0967-3849 4:8<583 1996 4 10577 https://doi.org/10.1007/BF02261721 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261721 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Richard USDA-ARS (United States Department of Agriculture-Agricultural Research Service) Forage and Range Research Laboratory, Utah State University, 84322-6300, Logan, UT, USA aut NATIONALLICENCE 700 1- Zhang Xue-Yong Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081, Beijing, China aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 583-587 0967-3849 4:8<583 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer