caa a22 4500 477089976 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02261720 doi (NATIONALLICENCE)springer-10.1007/BF02261720 Scanning ion analytical microscopy for high-resolution detection of 5-bromo-2′-deoxyuridine incorporation in metaphase chromosomes [Elektronische Daten] [C. Bourgeois, R. Dennebouy, A. Gibaud, M. Gerbault-Seureau, B. Malfoy, G. Slodzian, P. Galle, B. Dutrillaux] We investigated the possibilities of using scanning ion analytical microscopy (SIAM) to detect bromine in human metaphase chromosomes. The experiments were performed after incorporation of the thymidine analogue, 5-bromo-2′-deoxyuridine (BrdU), into the DNA or byin situ hybridization of a BrdU-labelled probe for the subcentromeric repeated DNA sequences. The possibilities offered by this microanalytical method were compared with immunofluorescent staining techniques. Well-defined maps of bands containing bromine were obtained with metaphase chromosomes that had incorporated BrdU during the late S-phase. Their patterns were similar to the labelling obtained by immunofluorescence. In addition, SIAM reveals the presence of bromine within constitutive heterochromatic regions in which BrdU is poorly detected by immunofluorescence. The comparison of the12C14N,31P and81Br maps of controls and fluorescence plus Giemsa (FPG) metaphase chromosomes, shows the loss of bromine from DNA during this treatment. SIAM emerges as a new powerful microanalytical technology for investigating chromosome structure further. Rapid Science Publishers, 1996 5-bromo-2′-deoxyuridine incorporation nationallicence chromosome structure nationallicence immunostaining nationallicence scanning ion microscopy nationallicence Bourgeois C. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut Dennebouy R. Laboratoire de Physique des Solides, Université Paris Sud, Orsay, France aut Gibaud A. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut Gerbault-Seureau M. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut Malfoy B. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut Slodzian G. Laboratoire de Physique des Solides, Université Paris Sud, Orsay, France aut Galle P. SC27 INSERM, Faculté de Médecine, 94010, Créteil, France aut Dutrillaux B. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 574-582 0967-3849 4:8<574 1996 4 10577 https://doi.org/10.1007/BF02261720 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261720 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bourgeois C. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Dennebouy R. Laboratoire de Physique des Solides, Université Paris Sud, Orsay, France aut NATIONALLICENCE 700 1- Gibaud A. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Gerbault-Seureau M. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Malfoy B. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Slodzian G. Laboratoire de Physique des Solides, Université Paris Sud, Orsay, France aut NATIONALLICENCE 700 1- Galle P. SC27 INSERM, Faculté de Médecine, 94010, Créteil, France aut NATIONALLICENCE 700 1- Dutrillaux B. Institut Curie-CNRS UMR 147, 26 rue d'Ulm, 75231, Paris Cedex 05, France aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 574-582 0967-3849 4:8<574 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer