caa a22 4500 477089984 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02261719 doi (NATIONALLICENCE)springer-10.1007/BF02261719 Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I human spermatocytes [Elektronische Daten] [Andrew Barlow, M. Hultén] We have used a combination of immunocytogenetic and molecular cytogenetic technology on human spermatocytes to investigate (1) melosis I chromosome pairing, and (2) organization of synaptonemal complex (SC)-associated chromatin with respect to whole chromosome paints, unique DNA sequences and repetitive DNA of heterochromatic blocks, centromeres and telomeres. It is evident that synapsis normally starts at the termini of homologues. In general, synapsis proceeds synchronously from termini towards the centre of bivalents without any indication of interstitial initiation. Some aberrant meiosis I spermatocytes showed asynchronous pairing, demonstrating not only large differences in the degree of SC formation between bivalents, but also chromosome alignment without synapsis as well as clear interstitial synaptic initiation. It may be the case that alignment normally takes place along the entire length of homologues before synapsis occurs and that the potential for synaptic initiation exists along the length of chromosomes. Telomeric sequences were seen tightly associated with the SCs, as might be expected considering their kinetic properties in relation to the nuclear membrane. Other repetitive DNA, i.e. centromeric α-satellites and classical satellites of the heterochromatic blocks 1qh and 9qh, were all found to form loops that are associated with SCs only at their bases. A unique DNA cosmid probe (21q22.3) was found to produce a hybridization pattern consisting of spots located outside SC. The fluorescencein situ hybridization (FISH) signals of these spread DNA sequences have a granular appearance, probably reflecting the pattern of coiling and chromatin condensation of the target DNAs. Rapid Science Publishers, 1996 human nationallicence immunocytology nationallicence in situ hybridization nationallicence meiosis nationallicence spermatocytes nationallicence synaptonemal complex nationallicence Barlow Andrew LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Yardley Green Road, B9 5PX, Birmingham, UK aut Hultén M. LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Yardley Green Road, B9 5PX, Birmingham, UK aut Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 562-573 0967-3849 4:8<562 1996 4 10577 https://doi.org/10.1007/BF02261719 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261719 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barlow Andrew LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Yardley Green Road, B9 5PX, Birmingham, UK aut NATIONALLICENCE 700 1- Hultén M. LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Yardley Green Road, B9 5PX, Birmingham, UK aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 562-573 0967-3849 4:8<562 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer