caa a22 4500 477089992 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02261723 doi (NATIONALLICENCE)springer-10.1007/BF02261723 Paternally inherited chromosomal structural aberrations detected in mouse first-cleavage zygote metaphases by multicolour fluorescence in situ hybridization painting [Elektronische Daten] [Francesco Marchetti, Xiu Lowe, Dan Moorell, Jack Bishop, Andrew Wyrobek] We describe a fluorescencein situ hybridization (FISH) procedure for assessing zygotic risk of paternal exposure to endogenous or exogenous agents. The procedure employs multicolour FISH with chromosome-specific DNA painting probes plus DAPI staining for detecting both balanced and unbalanced chromosomal aberrations in mouse first-cleavage (1-Cl) zygote metaphases. Four composite probes specific for chromosomes 1, 2, 3 or X, each labelled with biotin, plus a composite probe specific for chromosome Y labelled with digoxigenin, were used. We applied this method to evaluate the effects of paternal exposure to acrylamide, a model germ cell clastogen. First-cleavage zygote metaphases, collected from untreated females mated to males whose sperm or late spermatids were treated with acrylamide, were scored for the induction of structural aberrations using bothchromosome painting (PAINT analysis) andDAPI analysis. Structural chromosomal aberrations were observed in the sperm-deried, but not in the eggderived, pronuclei. While 59.4% of the zygotes had structural aberrations by DAPI analysis, 94.1% of the same zygotes had structural aberrations by PAINT analysis (P<0.001), illustrating the increased sensitivity for detecting translocations and insertions obtained by adding chromosome painting. These findings show that FISH painting of mouse 1-Cl zygotes when used in conjunction with DAPI analysis is a powerful model for investigating the cytogenetic defects transmitted from father to offspring. Rapid Science Publishers, 1996 acrylamide nationallicence chromosome painting nationallicence germ cell nationallicence translocation nationallicence Marchetti Francesco Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut Lowe Xiu Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut Moorell Dan Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut Bishop Jack National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA aut Wyrobek Andrew Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 604-613 0967-3849 4:8<604 1996 4 10577 https://doi.org/10.1007/BF02261723 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261723 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marchetti Francesco Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut NATIONALLICENCE 700 1- Lowe Xiu Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut NATIONALLICENCE 700 1- Moorell Dan Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut NATIONALLICENCE 700 1- Bishop Jack National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA aut NATIONALLICENCE 700 1- Wyrobek Andrew Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, PO Box 808, 94550, Livermore, CA, USA aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 604-613 0967-3849 4:8<604 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer