caa a22 4500 47709001X CHVBK 20180405111514.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF02261718 doi (NATIONALLICENCE)springer-10.1007/BF02261718 Ribosomal RNA location in the Antarctic fish Champsocephalus gunnari (Notothenioidei, Channichthyidae) using banding and fluorescence in situ hybridization [Elektronische Daten] [C. Ozouf-Costaz, E. Pisano, C. Bonillo, R. Williams] A biotinylated 28S rDNA probe was prepared from the genomic DNA of the Antarctic ice-fishChampsocephalus gunnari and hybridized to metaphase chromosomes of the same species by fluorescencein situ hybridization (FISH). The hybridization signal appeared over the whole heterochromatic arm of the submetacentric chromosomes bearing the nucleolar organizer regions. The results of rDNA/FISH are compared with those coming from classical cytogenetic (C, Q, Ag-NOR, chromomycin A3) banding techniques. Thein situ detection of a specific DNA sequence offers a new more precise perspective for understanding the evolving process in chromosomes of Antarctic fish and will provide an interesting contribution to comparative cytogenetics of lower vertebrates. Rapid Science Publishers, 1996 Antarctic fish nationallicence Champsocephalus gunnari nationallicence Channichthyidae nationallicence fluorescence in situ hybridization nationallicence molecular cytogenetics nationallicence rDNA nationallicence Ozouf-Costaz C. Muséum National d'Histoire Naturelle, Laboratoire d'Ichthyologie, CNRS GDR 1005, 43 rue Cuvier, 75231, Paris Cedex 05, France aut Pisano E. Muséum National d'Histoire Naturelle, Laboratoire d'Ichthyologie, CNRS GDR 1005, 43 rue Cuvier, 75231, Paris Cedex 05, France aut Bonillo C. Institute of Comparative Anatomy, University of Genoa, Viale Benedetto XV, 5, 16132, Genoa, Italy aut Williams R. Australian Antarctic Division, Channel Highway, Kingston, Tasmania, Australia aut Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 557-561 0967-3849 4:8<557 1996 4 10577 https://doi.org/10.1007/BF02261718 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261718 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ozouf-Costaz C. Muséum National d'Histoire Naturelle, Laboratoire d'Ichthyologie, CNRS GDR 1005, 43 rue Cuvier, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Pisano E. Muséum National d'Histoire Naturelle, Laboratoire d'Ichthyologie, CNRS GDR 1005, 43 rue Cuvier, 75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Bonillo C. Institute of Comparative Anatomy, University of Genoa, Viale Benedetto XV, 5, 16132, Genoa, Italy aut NATIONALLICENCE 700 1- Williams R. Australian Antarctic Division, Channel Highway, Kingston, Tasmania, Australia aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/8(1996-12-01), 557-561 0967-3849 4:8<557 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer