caa a22 4500 477090036 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02257271 doi (NATIONALLICENCE)springer-10.1007/BF02257271 The Ty1-copia group retrotransposons of Allium cepa are distributed throughout the chromosomes but are enriched in the terminal heterochromatin [Elektronische Daten] [Stephen Pearce, Uta Pich, Gill Harrison, Andrew Flavell, J. Heslop-Harrison, Ingo Schubert, Amar Kumar] The genomic organization and diversity of theTy1-copia group retrotransposons has been investigated in a monocotyledonous plant,Allium cepa. We used the polymerase chain reaction (PCR) to generate sequences corresponding to a conserved domain of the reverse transcriptase gene ofTy1-copia retrotransposons in this plant. Sequence analysis of 27 of these PCR products shows that they are a highly heterogeneous population, a feature which is common in plants but not in yeast andDrosophila. Slot-blot analysis shows there are 100 000-200 000 copies ofTy1-copia group retrotransposons within theA. cepa genome (2C=31.7 pg), indicating that they are a significant component of the genome of this plant.In situ hybridization to metaphase chromosomes reveals thatTy1-copia retrotransposons are distributed throughout the euchromatin of all chromosomes ofA. cepa but are enriched in the terminal heterochromatic regions, which contain tandem arrays of satellite sequences. This is the first clear evidence for the presence ofTy1-copia retrotransposons in the terminal heterochromatin of plants and contrasts with the distribution of these elements in other plant species. Rapid Science Publishers, 1996 heterochromatin nationallicence onion nationallicence plants nationallicence retrotransposons nationallicence Ty1-copia nationallicence Pearce Stephen Cell and Molecular Genetics Department, Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut Pich Uta Institute of Plant Genetics and Crop Plant Research, Corrensstr 3, D-06466, Gatersleben, Germany aut Harrison Gill Karyobiology Group, John Innes Centre, NR4 7UH, Colney Lane, Norwich, UK aut Flavell Andrew Department of Biochemistry, University of Dundee, DD1 4HN, UK aut Heslop-Harrison J. Karyobiology Group, John Innes Centre, NR4 7UH, Colney Lane, Norwich, UK aut Schubert Ingo Institute of Plant Genetics and Crop Plant Research, Corrensstr 3, D-06466, Gatersleben, Germany aut Kumar Amar Cell and Molecular Genetics Department, Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 357-364 0967-3849 4:5<357 1996 4 10577 https://doi.org/10.1007/BF02257271 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02257271 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pearce Stephen Cell and Molecular Genetics Department, Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut NATIONALLICENCE 700 1- Pich Uta Institute of Plant Genetics and Crop Plant Research, Corrensstr 3, D-06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Harrison Gill Karyobiology Group, John Innes Centre, NR4 7UH, Colney Lane, Norwich, UK aut NATIONALLICENCE 700 1- Flavell Andrew Department of Biochemistry, University of Dundee, DD1 4HN, UK aut NATIONALLICENCE 700 1- Heslop-Harrison J. Karyobiology Group, John Innes Centre, NR4 7UH, Colney Lane, Norwich, UK aut NATIONALLICENCE 700 1- Schubert Ingo Institute of Plant Genetics and Crop Plant Research, Corrensstr 3, D-06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Kumar Amar Cell and Molecular Genetics Department, Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 357-364 0967-3849 4:5<357 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer