caa a22 4500 477090079 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02257275 doi (NATIONALLICENCE)springer-10.1007/BF02257275 Chromosomal localization of a highly repeated Eco RI DNA fragment in Megoura viciae (Homoptera, Aphididae) by nick translation and fluorescence in situ hybridization [Elektronische Daten] [Davide Bizzaro, Gian Manicardi, Umberto Bianchi] To investigate the genome of the aphidMegoura viciae at molecular level, we have studied total DNA by agarose gel electrophoresis after cleavage with different restriction endonucleases.EcoRI digestion produced a highly repeated DNA fragment, about 600 bp long. The contribution of thisEcoRI element to the total genome ofM. viciae was estimated at about 6% by means of densitometric scanning of agarose gel photographs. The chromosomal localization of this fragment, investigated by fluorescentin situ hybridization (FISH), constantly showed one large and two narrower fluorescent bands located on the X chromosome, all corresponding to C-positive heterochromatic areas. These results are in full accordance with the data obtained byin situ nick translation experiments carried out afterEcoRI digestion, and clearly demonstrate that a substantial amount ofM. viciae heterochromatin consists ofEcoRI fragments which are mainly located on the X chromosome. Using theEcoRI restriction fragment as a molecular probe may prove to be a practical tool for the investigation of taxonomic and evolutionary relationships in this group of insects. Rapid Science Publishers, 1996 aphids nationallicence DNA satellite nationallicence fluorescence in situ hybridization nationallicence heterochromatin nationallicence holocentric chromosomes nationallicence Bizzaro Davide Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut Manicardi Gian Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut Bianchi Umberto Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 392-396 0967-3849 4:5<392 1996 4 10577 https://doi.org/10.1007/BF02257275 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02257275 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bizzaro Davide Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Manicardi Gian Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Bianchi Umberto Dipartimento di Biologia Animale, Università di Modena, Viale Berengario 14, 41100, Modena, Italy aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 392-396 0967-3849 4:5<392 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer