caa a22 4500 477090109 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02257272 doi (NATIONALLICENCE)springer-10.1007/BF02257272 Genomic instability in MycER-activated Rat1A-MycER cells [Elektronische Daten] [Sabine Mai, Monika Fluri, David Siwarski, Konrad Huppi] The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged periods. To this end, we have used Rat1A-MycER cells, which allow the experimental regulation of Myc protein levels. We examined the genomic stability of Rat1A-MycER cells cultivated in either the absence or the presence of estrogen, which reportedly activates the chimeric MycER protein in these cells. Following prolonged periods of MycER activation, Rat1A-Mycer cells exhibited irreversible chromosomal aberrations. The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements. Rapid Science Publishers, 1996 Mai Sabine Manitoba Institute of Cell Biology, 100 Olivia Street, R3E 0V9, Winnipeg, Manitoba, Canada aut Fluri Monika Basle Institute for Immunology, Grenzacherstr. 487, CH-4005, Basle, Switzerland aut Siwarski David Molecular Genetics Section, Laboratory of Genetics, NCI/NIH, Building 37, Room 2B21, 20892, Bethesda, Maryland, USA aut Huppi Konrad Molecular Genetics Section, Laboratory of Genetics, NCI/NIH, Building 37, Room 2B21, 20892, Bethesda, Maryland, USA aut Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 365-371 0967-3849 4:5<365 1996 4 10577 https://doi.org/10.1007/BF02257272 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02257272 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mai Sabine Manitoba Institute of Cell Biology, 100 Olivia Street, R3E 0V9, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Fluri Monika Basle Institute for Immunology, Grenzacherstr. 487, CH-4005, Basle, Switzerland aut NATIONALLICENCE 700 1- Siwarski David Molecular Genetics Section, Laboratory of Genetics, NCI/NIH, Building 37, Room 2B21, 20892, Bethesda, Maryland, USA aut NATIONALLICENCE 700 1- Huppi Konrad Molecular Genetics Section, Laboratory of Genetics, NCI/NIH, Building 37, Room 2B21, 20892, Bethesda, Maryland, USA aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 365-371 0967-3849 4:5<365 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer