caa a22 4500 477090117 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02257269 doi (NATIONALLICENCE)springer-10.1007/BF02257269 Evidence for a sequence-directed conformation periodicity in the genomic highly repetitive DNA detectable with single-strand-specific chemical probe potassium permanganate [Elektronische Daten] [Roman Matyášek, Jaroslav Fulneček, Jiří Fajkus, Milan Bezděk] A single-strand-specific chemical probe, potassium permanganate (KMnO4), was used to study the sequence-dependent conformation periodicity of tandem multicopy repetitive DNA sequences HRS60 and GRS (Nicotiana species) at the level of single base pair and dinucleotide step. Local DNA structures, sensitive to KMnO4, revealed periodicity of 182±2 bp, equal to the length of repeat units. Permanganate-sensitive local structures were mapped in both DNA strands of genomic HRS60 sequences and were found to be linked to d(A)n tracts. These adenine tracts are located in the proximity of the intrinsically curved domains. Distamycin A increased reactivity of the DNA but decreased the specificity of DNA cleavage. Similar conformation periodicity has been detected also in the ‘canrep' family of repeats (Brassica species). All studied repetitive sequences are predominantly located in the constitutive heterochromatin. We discuss the role of conformation periodicties in relation to a structural code for nucleosome phasing at tandem arrays of DNA repeats. Rapid Science Publishers, 1996 chromosome structure nationallicence DNA conformation nationallicence nucleosomes nationallicence tobacco nationallicence Matyášek Roman Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut Fulneček Jaroslav Department of Biochemistry, Masaryk University, CZ-61137, Brno, Czech Republic aut Fajkus Jiří Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut Bezděk Milan Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 340-349 0967-3849 4:5<340 1996 4 10577 https://doi.org/10.1007/BF02257269 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02257269 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matyášek Roman Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut NATIONALLICENCE 700 1- Fulneček Jaroslav Department of Biochemistry, Masaryk University, CZ-61137, Brno, Czech Republic aut NATIONALLICENCE 700 1- Fajkus Jiří Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut NATIONALLICENCE 700 1- Bezděk Milan Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/5(1996-08-01), 340-349 0967-3849 4:5<340 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer