caa a22 4500 477090257 CHVBK 20180405111514.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02259702 doi (NATIONALLICENCE)springer-10.1007/BF02259702 Post-mitotic assembly of the nucleolus. I. The internal matrix network is a recruitment site for processing nucleolar components in prenucleolar bodies [Elektronische Daten] [Susana Moreno Díaz de la Espina, Ana Minguez] The aim of this work was to investigate whether the nuclear matrix could provide the nucleation sites for dispersed parental nucleolar components to form post-mitotic prenucleolar bodies (PNBs). For this purpose, nuclear matrices from asynchronous populations of onion cells were fractionated, and the distribution of the insoluble components of the nucleolar processing complexes in the matrices were analysed by fibrillarin immunolabelling. The ultrastructural organization of the nuclear matrix of cells from late telophase to late G1, corresponding to the period of nucleolar reassembly and activation, was also analysed. Our results demonstrate that PNBs are structural components of the telophasic nuclear matrix and that this structure provides recruitment and assembly sites for the components of the nucleolar processing machinery, and suggests that the telophasic matrix network is involved in the early steps of post-mitotic nucleologenesis. Rapid Science Publishers, 1996 fibrillarin nationallicence nuclear matrix nationallicence onion nationallicence prenucleolar bodies nationallicence Moreno Díaz de la Espina Susana Laboratorio de Biología Celular y Molecular Vegetal, Centro Investigaciones Biológicas, CSIC, Velázquez, 144, E-28006, Madrid, Spain aut Minguez Ana Laboratorio de Biología Celular y Molecular Vegetal, Centro Investigaciones Biológicas, CSIC, Velázquez, 144, E-28006, Madrid, Spain aut Chromosome Research Kluwer Academic Publishers 4/2(1996-03-01), 103-110 0967-3849 4:2<103 1996 4 10577 https://doi.org/10.1007/BF02259702 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02259702 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moreno Díaz de la Espina Susana Laboratorio de Biología Celular y Molecular Vegetal, Centro Investigaciones Biológicas, CSIC, Velázquez, 144, E-28006, Madrid, Spain aut NATIONALLICENCE 700 1- Minguez Ana Laboratorio de Biología Celular y Molecular Vegetal, Centro Investigaciones Biológicas, CSIC, Velázquez, 144, E-28006, Madrid, Spain aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/2(1996-03-01), 103-110 0967-3849 4:2<103 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer