caa a22 4500 477095089 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/s004290050027 doi (NATIONALLICENCE)springer-10.1007/s004290050027 Phenotype of cultured fetal perivascular cells from human placenta studied by scanning electron microscopy [Elektronische Daten] [J. C. Challier, A. Kacemi, M. Galtier, M. Boucher, M. J. Tangapregassom, C. Vervelle, T. Bintein, M. J. Espié, G. Olive]  The phenotype of perivascular placental cells has previously been studied using tissue sections from the fetal villi. The examination of these cells in culture by scanning electron microscopy gives us the opportunity to observe their three-dimensional phenotypes and associations outside their normal constraints. Human umbilical endothelial cells, which have a phenotype comparable to that observed in other sutdies, seem more flattened in culture than in their usual environment. Microvascular endothelial cells did not attain an epithelioid phenotype with close contacts between cells but formed a network of branched, elongated cells with phagocytotic activity. Some circular associations were observed when using a gelatinized matrix. Microvascular pericytes were large, flattened cells with an irregular border that pushed up nodular associations on a gelatin matrix. Chorioplacental myocytes adopted a network template comparable to that developed by microvascular endothelial cells. However, these elongated cells were thicker, without microvilli, and superficial filaments could be observed. In culture, confluent endothelial cells from the umbilical cord or microvascular pericytes associated as nodules reached a cell phenotype close to their in vivo counterparts. This attainment of an in vivo phenotype remains questionable for chorioplacental myocytes. Microvascular endothelial cells, however, though there was sparse formation of circular associations, remained far from their in vivo phenotype. Springer-Verlag Berlin Heidelberg, 1996 Key words Human placenta nationallicence Pericytes nationallicence Endothelial cells nationallicence Myocytes nationallicence Culture nationallicence Scanning electron microscopy nationallicence Phenotype nationallicence Challier J. C. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Kacemi A. Université René Descartes, CHU-Cochin Port-Royal, Pharmacologie Biochimique, Gustave Roussy, 24 Rue du Faubourg St. Jacques, F-75014 Paris, France, FR aut Galtier M. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Boucher M. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Tangapregassom M. J. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Vervelle C. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Bintein T. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Espié M. J. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut Olive G. Université René Descartes, CHU-Cochin Port-Royal, Pharmacologie Biochimique, Gustave Roussy, 24 Rue du Faubourg St. Jacques, F-75014 Paris, France, FR aut https://doi.org/10.1007/s004290050027 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004290050027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Challier J. C. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Kacemi A. Université René Descartes, CHU-Cochin Port-Royal, Pharmacologie Biochimique, Gustave Roussy, 24 Rue du Faubourg St. Jacques, F-75014 Paris, France, FR aut NATIONALLICENCE 700 1- Galtier M. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Boucher M. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Tangapregassom M. J. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Vervelle C. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Bintein T. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Espié M. J. Université Pierre et Marie Curie, Physiopathologie du Développement, 4 place Jussieu, F-75252 Paris Cedex 05, France Tel.: +33.01.44.27.58.47; Fax: +33.01.44.27.58.48; e-mail: Jean-Claude.Challier@snv.jussieu.fr, FR aut NATIONALLICENCE 700 1- Olive G. Université René Descartes, CHU-Cochin Port-Royal, Pharmacologie Biochimique, Gustave Roussy, 24 Rue du Faubourg St. Jacques, F-75014 Paris, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer