caa a22 4500 477095119 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/s004290050022 doi (NATIONALLICENCE)springer-10.1007/s004290050022 Expression and localization of annexin V and annexin VI during limb bud formation in the rat fetus [Elektronische Daten] [Mohammed Mohibur Rahman, Hiroshi Iida, Yosaburo Shibata]  We have investigated the expression and the localization of annexin V and annexin VI during the development of rat fetal limb buds by immunoblot and immunocytochemical analysis. Neither annexin V nor annexin VI was detectable in undifferentiated mesenchymal cells in limb buds of the day-13-day-16 rat fetus. Skeletal muscles, whose progenitor cells migrate from the somites and appeared in the limb buds at day 14, dramatically expressed annexin VI on the cell surface after differentiation from mononucleated myogenic cells into multinucleated myotubes. At day 16 both annexin V and annexin VI were found to be expressed in differentiated chondrocytes as well as in the perichondrium, a precursor of chondrocytes, whereas the compact layer of mesenchymal cells surrounding a chondrification center (precartilage) did not show any immunoreactivity for either of these proteins. The results suggest a close relationship between the expression of these annexins and cell differentiation of chondrocytes and skeletal muscles during limb but development. Springer-Verlag Berlin Heidelberg, 1996 Key words Chondrocyte nationallicence Skeletal muscle nationallicence Calcium-binding protein nationallicence Immunohistochemistry nationallicence Rahman Mohammed Mohibur Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut Iida Hiroshi Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut Shibata Yosaburo Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut https://doi.org/10.1007/s004290050022 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004290050022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rahman Mohammed Mohibur Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut NATIONALLICENCE 700 1- Iida Hiroshi Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut NATIONALLICENCE 700 1- Shibata Yosaburo Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka 812-82, Japan Tel.: 92-641-1151 ext. 3267; Fax: 92-632-2373, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer