caa a22 4500 477095194 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00186834 doi (NATIONALLICENCE)springer-10.1007/BF00186834 Meckel's cartilage chondrocytes in organ culture synthesize bone-type proteins accompanying osteocytic phenotype expression [Elektronische Daten] [Kiyoto Ishizeki, Masaharu Takigawa, Yorio Harada, Fujio Suzuki, Tokio Nawa] We examined whether Meckel's cartilage of embryonic mice, 17 days in utero, undergo the cellular transformation into the osteocyte-like phenotype under organ culture conditions. Explants were grown by our original pithole method modified Trowell-type cultures for up to 4 weeks at 37° C under 5% CO2 in air. Specimens were examined using histological procedures including immunostaining and electron microscopy. In addition, the effects of β-glycerophosphate on matrix calcification were also examined in cultures with or without β-glycerophosphate. Addition of β-glycerophosphate induced calcification at a higher level, but calcium mineral deposition occurred regardless of the addition of β-glycerophosphate to the culture medium. Light and electron microscopic analyses showed that freshly isolated chondrocytes prior to cell culture had typical hypertrophic morphology, but shortly after commencement of culture, they showed morphological modifications. The cells showing chondrocytic phenotypes became basophilic elliptical cells, and eventually transformed into flattened osteocyte-like cells. Bone-like features for cellular elements were characterized by spindle-shaped cells with elongated processes accompanying bone-specific thickbanded collagen fibrils. Immunostaining showed that at 2 weeks in culture, type I and type II collagens coexisted in the matrix, but subsequently type II collagen synthesis ceased and was replaced by type I collagen synthesis. Immunofluorescent labeling for osteocalcin was noted first in the peripheral cells by 1 week, but at 3 weeks this reaction spread to the central zone in explants. Alkaline phosphatase activity (ALPase) was expressed on the cells in the central zone prior to calcium mineral deposition as shown by von Kossa's reaction at 3 weeks in culture. These results showed that Meckel's cartilage chondrocytes in organ culture synthesize bone-type proteins accompanying osteocytic phenotype expression. Springer-Verlag, 1996 Meckel's cartilage nationallicence Organ culture nationallicence Transformation nationallicence Calcification nationallicence Osteocyte-like cell nationallicence Ishizeki Kiyoto Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut Takigawa Masaharu Department of Biochemistry, Okayama University Dental School, Sikatacho, 700, Okayama, Japan aut Harada Yorio Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut Suzuki Fujio Department of Biochemistry and Calcified-Tissue Metabolism, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, 565, Suita Osaka, Japan aut Nawa Tokio Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut Anatomy and Embryology Springer-Verlag 193/1(1996-01-01), 61-71 0340-2061 193:1<61 1996 193 429 https://doi.org/10.1007/BF00186834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00186834 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishizeki Kiyoto Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut NATIONALLICENCE 700 1- Takigawa Masaharu Department of Biochemistry, Okayama University Dental School, Sikatacho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Harada Yorio Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut NATIONALLICENCE 700 1- Suzuki Fujio Department of Biochemistry and Calcified-Tissue Metabolism, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, 565, Suita Osaka, Japan aut NATIONALLICENCE 700 1- Nawa Tokio Department of Oral Anatomy, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, 020, Morioka, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/1(1996-01-01), 61-71 0340-2061 193:1<61 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer