caa a22 4500 477095216 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00187932 doi (NATIONALLICENCE)springer-10.1007/BF00187932 The effect of embryonic cerebrospinal fluid pressure and morphogenetic brain expansion on wound healing in the midbrain of the chick embryo [Elektronische Daten] [Aaron Lawson, Marjorie England] The role of increased cerebrospinal fluid pressure and morphogenetic brain expansion on midbrain wound healing was studied in chick embryos at stages 16-22. The embryos were divided into six groups as follows: group I (stages 16/17), group II (stages 18/19), group III (stages 20-22), group IV (stages 18/19), group V (stages 20-22) and group VI (stages 18/19). The midbrains of embryos of groups I-III were wounded and the embryos re-incubated for varying periods up to 24 h. The neuroepithelial wounds of all group-I embryos healed completely within 24 h. However, complete healing was observed in only 25% of wounds in group II and 11.4% in group III by 24 h. To reduce cerebrospinal fluid pressure and thus slow down brain expansion, longitudinal wounds (about 0.8 mm long) were made in the hindbrain roof plate of group-IV and group-V embryos, and puncture wounds (0.1 mm in diameter) also in the hindbrain roof plate of group-VI embryos. This allowed cerebrospinal fluid to escape prior to wounding the midbrain. There was a significant increase in the proportion of group-IV and group-V embryos with completely healed midbrain neuroepithelial wounds (77.3% and 28.6% respectively). However, a comparison between groups II and VI embryos yielded no statistically significant difference in healing. Thus, increasing cerebrospinal fluid pressure and brain expansion adversely affect midbrain neuroepithelial wound healing. Springer-Verlag, 1996 Neuroepithelium nationallicence Surface ectoderm nationallicence Cerebrospinal fluid pressure nationallicence Morphogenesis nationallicence Neural tube defects nationallicence Lawson Aaron Department of Anatomy, University of Ghana Medical School, P.O. Box 4236, Accra, Ghana aut England Marjorie Department of Pre-Clinical Sciences, University of Leicester, University Road, P.O. Box 138, LE1 9HN, Leicester, UK aut Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 601-610 0340-2061 193:6<601 1996 193 429 https://doi.org/10.1007/BF00187932 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187932 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lawson Aaron Department of Anatomy, University of Ghana Medical School, P.O. Box 4236, Accra, Ghana aut NATIONALLICENCE 700 1- England Marjorie Department of Pre-Clinical Sciences, University of Leicester, University Road, P.O. Box 138, LE1 9HN, Leicester, UK aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 601-610 0340-2061 193:6<601 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer