caa a22 4500 477095240 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00187925 doi (NATIONALLICENCE)springer-10.1007/BF00187925 In vivo inhibition of programmed cell death by local administration of FGF-2 and FGF-4 in the interdigital areas of the embryonic chick leg bud [Elektronische Daten] [Domingo Macias, Yolanda Gañan, Maria Ros, Juan Hurle] The formation of the digits in amniote vertebrates is accompanied by a massive degeneration process that accounts for the disappearance of the interdigital mesenchyme. The establishment of these areas of interdigital cell death (INZs) is concomitant with the flattening of the apical ectodermal ridge (AER), but a possible causal relationship between these processes has not been demonstrated. Recent studies have shown that the function of the AER can be substituted for by implantation of beads bearing either FGF-2 or FGF-4 into the apical mesoderm of the early limb bud. According to these observations, if the onset of INZs is triggered by the cessation of the AER function, local administration of FGFs to the interdigital tissue prior to cell death should delay or inhibit interdigit degeneration. In the present study we have confirmed this prediction. Implanting Affi-gel blue or heparin beads pre-absorbed with either FGF-2 or FGF-4 into the interdigital tissue of the chick leg bud in the stages prior to cell death stimulates cell proliferation and causes the formation of webbed digits. Vital staining with neutral red confirmed an intense temporal inhibition of interdigital cell death after FGF treatment. This inhibition of interdigital cell death was not accompanied by modifications in the pattern of expression of Msx-1 or Msx-2 genes, which in normal development display a domain of expression in the interdigital tissue preceding the onset of degeneration. Springer-Verlag, 1996 Apoptosis nationallicence Limb morphogenesis nationallicence Growth factors nationallicence Macias Domingo Departamento de Ciencias Morfológicas, Universidad de Extremadura, E-06071, Badajoz, Spain aut Gañan Yolanda Departamento de Ciencias Morfológicas, Universidad de Extremadura, E-06071, Badajoz, Spain aut Ros Maria Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut Hurle Juan Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 533-541 0340-2061 193:6<533 1996 193 429 https://doi.org/10.1007/BF00187925 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187925 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Macias Domingo Departamento de Ciencias Morfológicas, Universidad de Extremadura, E-06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Gañan Yolanda Departamento de Ciencias Morfológicas, Universidad de Extremadura, E-06071, Badajoz, Spain aut NATIONALLICENCE 700 1- Ros Maria Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut NATIONALLICENCE 700 1- Hurle Juan Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 533-541 0340-2061 193:6<533 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer