caa a22 4500 477095305 CHVBK 20180405111525.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00187933 doi (NATIONALLICENCE)springer-10.1007/BF00187933 Ultrastructural localization of NADPH-diaphorase activity in the submucous ganglia of the guinea-pig intestine after vagotomy [Elektronische Daten] [X. Wang, W. Wong, E. Ling] The reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity in the axon terminals presynaptic to the submucous neurons of guinea-pig intestine following unilateral cervical vagotomy was studied by electron microscopy. The reaction product of diaphorase was localized only in the axon terminals that contained predominantly small agranular vesicles, and it was usually deposited around the vesicles. The terminals that contained predominantly large granular or flattened vesicles did not display any signs of diaphorase reactivity. Although there were only few diaphorase-positive submucous neurons in the small intestine, a considerable number of diaphorase-positive axon terminals was observed in the submucous ganglia of the small intestine in the control animals. Ten days after vagotomy, the quantitative study showed that when compared with the control animals, the number of diaphorase-positive terminals in the submucous ganglia of duodenum, mid-small intestine and colon in the vagotomized animals was reduced (P<0.05). When the NADPHd-positive terminals were examined in closer detail, it was found that only a small proportion of them showed signs of degeneration as evidenced by the swelling and vacuolation of their contents of mitochondria, with disrupted cristae and clumping of synaptic vesicles. It was therefore concluded that at least some of the diaphorasepositive axon terminals in the submucous ganglia of guinea-pig intestine originated from the vagus nerve. Springer-Verlag, 1996 Guinea-pig nationallicence Intestine nationallicence Submucous ganglia nationallicence NADPH-diaphorase nationallicence Vagotomy nationallicence Wang X. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut Wong W. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut Ling E. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 611-617 0340-2061 193:6<611 1996 193 429 https://doi.org/10.1007/BF00187933 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187933 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang X. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut NATIONALLICENCE 700 1- Wong W. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut NATIONALLICENCE 700 1- Ling E. Department of Anatomy, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/6(1996-06-01), 611-617 0340-2061 193:6<611 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer