caa a22 4500 47709533X CHVBK 20180405111525.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00198326 doi (NATIONALLICENCE)springer-10.1007/BF00198326 Quantitative electromicroscope study of the oculomotor parasympathetic neurons projecting to the ciliary ganglion in cats: comparison of the synaptic (axo-somatic and axo-proximal dendritic) organization of anterior-dorsal and ventral cell groups [Elektronische Daten] [Noritaka Ichinohe, Kazuhiko Shoumura, Hitoshi Takahashi] The synaptic organization of the oculomotor parasympathetic preganglionic neurons (OPNs), labeled retrogradely after a horseradish peroxidase (HRP) injection into the ciliary ganglion, was studied in cats by electron microscopy. We divided the OPNs into two groups, anterior-dorsal (ADG) and ventral (VG) cell groups, based upon physiological studies in cats suggesting that accomodation-related OPNs are predominantly located anterior and dorsal to the somatic nuclei of the oculomotor nuclear complex (i.e., the anteromedian and Edinger Westphal nuclei, and the ventral central gray area), while pupillo-constriction-related OPNs are predominantly located ventral to the somatic nuclei (i.e., the ventral tegmental area). The synaptic organization of these two groups was quantitatively compared, using a nested analysis of variance to determine statistical significance (P<0.05). Partial reconstructions of the labeled somata and proximal dendrites were made from tracings of electron micrographs of every 2nd section in serial ultrathin sections that included the nucleolus or were adjacent to sections that included the nucleolus. The mean number of boutons of apposition on a reconstructed labeled soma of VG was significantly greater than that of ADG (mean ±SD; ADG, 5.3±3.3; VG, 8.6±3.2). The mean synaptic density on a VG soma was significantly greater than on an ADG soma (mean±SD; ADG, 3.74±2.11 counts/100 (μm2; VG, 6.30±1.99 counts/100 μm2). The mean synaptic covering ratio on a VG soma was significantly greater than on an ADG soma (mean±SD; ADG, 5.21±2.91%; VG, 10.14±3.76%). The mean estimated number of boutons of apposition on a VG soma was significantly greater than on an ADG soma (mean±SD: ADG, 53±36; VG, 100±48). Boutons were classified on the basis of the shape of their synaptic vesicles as S-type (containing spherical clear synaptic vesicles) or P-type (containing both flattened and spherical clear synaptic vesicles). The mean S-type/S+P-type bouton ratio on a VG soma was significantly greater than on an ADG soma (mean±SD; ADG, 0.31±0.20; VG, 0.67±0.18). The differences demonstrated in this study reinforce, morphologically, the assumption of functional localization of OPNs, and further allow us to estimate the relative characteristics of the synaptic organization of accommodation-related OPNs and pupillo-constriction-related OPNs. Springer-Verlag, 1996 Preganglionic neuron nationallicence Oculomotor nerve nationallicence Parasympathetic nervous system nationallicence Synapse nationallicence Ultrastructure nationallicence Ichinohe Noritaka Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut Shoumura Kazuhiko Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut Takahashi Hitoshi Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut Anatomy and Embryology Springer-Verlag 193/3(1996-03-01), 229-238 0340-2061 193:3<229 1996 193 429 https://doi.org/10.1007/BF00198326 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198326 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ichinohe Noritaka Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Shoumura Kazuhiko Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Takahashi Hitoshi Department of Anatomy, Hirosaki University School of Medicine, 5 Zaifucho, 036, Hirosaki, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/3(1996-03-01), 229-238 0340-2061 193:3<229 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer