caa a22 4500 477095399 CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00198328 doi (NATIONALLICENCE)springer-10.1007/BF00198328 Synaptic organization of afferent projections to the supramammillary nucleus of the rat [Elektronische Daten] [Tetsu Hayakawa, Katuya Zyo] We studied the fine structure of afferent terminals from the preoptic area, the nucleus of the diagonal band of Broca, the infralimbic cortex and the laterodorsal tegmental nucleus within the supramammillary nucleus (SUM) using the anterograde tracing method of horse-radish peroxidase conjugated with wheat germ agglutinin (WGA-HRP). Injection of WGA-HRP into the preoptic area permitted ultrastructural recognition of many anterogradely labeled terminals in the SUM. Almost all labeled terminals (99%) contained clear round synaptic vesicles and formed asymmetric synaptic contacts (Gray's type I). About 86% of labeled terminals from the nucleus of the diagonal band were asymmetric (Gray's type I), whereas 14% contained pleomorphic synaptic vesicles and formed symmetric synaptic contacts (Gray's type II). Almost all labeled terminals from the infralimbic cortex were located in the ventral part of the SUM, and 95% of labeled terminals were Gray's type I. The majority of labeled terminals (90%) from the laterodorsal tegmental nucleus were Gray's type I, and the remaining (10%) were Gray's type II. The percentage of labeled terminals with dense-cored vesicles was very high in terminals from the preoptic area (70%), and low in terminals from the infralimbic cortex (19%). Labeled terminals in all cases contacted mainly intermediate-sized dendrites (0.5-1.0 μm diameter). All cases had only a few labeled axosomatic terminals. The cases of injections into the preoptic area and the diagonal band nucleus had some reciprocal connections at the ultrastructural level. Springer-Verlag, 1996 WGA-HRP nationallicence anterograde tracing nationallicence Ultrastructure nationallicence Preoptic area nationallicence Nucleus of diagonal band nationallicence Laterodorsal tegmental nucleus nationallicence Hayakawa Tetsu Department of Anatomy, Hyogo College of Medicine, Mukogawa-cho, Nishinomiya, 663, Hyogo, Japan aut Zyo Katuya Department of Anatomy, Hyogo College of Medicine, Mukogawa-cho, Nishinomiya, 663, Hyogo, Japan aut Anatomy and Embryology Springer-Verlag 193/3(1996-03-01), 249-257 0340-2061 193:3<249 1996 193 429 https://doi.org/10.1007/BF00198328 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198328 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayakawa Tetsu Department of Anatomy, Hyogo College of Medicine, Mukogawa-cho, Nishinomiya, 663, Hyogo, Japan aut NATIONALLICENCE 700 1- Zyo Katuya Department of Anatomy, Hyogo College of Medicine, Mukogawa-cho, Nishinomiya, 663, Hyogo, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/3(1996-03-01), 249-257 0340-2061 193:3<249 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer