caa a22 4500 477095437 CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00186692 doi (NATIONALLICENCE)springer-10.1007/BF00186692 Extracellular matrix modifications in the interdigital spaces of the chick embryo leg bud during the formation of ectopic digits [Elektronische Daten] [Juan Hurle, Alfonso Colombatti] In previous studies we have observed that the interdigital mesenchyme of the chick leg bud, in the stages preceding the onset of cell death, retains a significant regulatory potential, forming ectopic extra digits under a variety of surgical manipulations. Most evidence suggests that interdigital extra digits are caused by the abolition of local antichondrogenic effects operating in the interdigital spaces under normal conditions rather than by modifications of the signalling mechanisms accounting for the normal patterning of the digits in early stages of development. The interdigital spaces exhibit a complex scaffold of extracellular matrix with well-defined domains of spatial distribution of type I and type VI collagens, tenascin, fibronectin, laminin and elastic matrix components that have been proposed to play a role in the establishment of the non-chondrogenic fate of the interdigital tissue in situ. In an attempt to analyze this possible role of the interdigital extracellular matrix (ECM), in the present work we have studied changes in the pattern of ECM distribution associated with the formation of extra digits. Extra digits were induced by making a T-cut in the third interdigital space of the leg but of stage 29 HH chick embryos. Subsequent modifications of the ECM were detected immunohistochemically in whole-mount specimens using laser confocal microscopy. Our results reveal that in the first hours after the operation, changes in the ECM apparently related to the healing of the wound cause a significant reorganization of the normal ECM scaffold of the interdigit. In addition, chondrogenesis of the interdigital tissue is preceded by disappearance of elastin fibers in the interdigital mesenchyme subjacent to the wound and by an intense deposition of tenascin. Tenascin deposition and loss of the elastin fibrillar scaffold were also observed preceding chondrogenesis in fragments of interdigital tissue explanted to culture conditions. The significance of these observations in relation to the establishment of the skeletal elements of the autopodium is discussed. Springer-Verlag, 1996 Limb morphogenesis nationallicence Elastic matrix nationallicence Tenascin nationallicence Fibronectin nationallicence Collagen type VI nationallicence Chondrogenesis nationallicence Hurle Juan Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut Colombatti Alfonso Divisone di Oncologia, Centro di Riferimento Oncologico, Sperimentale 2, I-33081, Aviano, Italy aut Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 355-364 0340-2061 193:4<355 1996 193 429 https://doi.org/10.1007/BF00186692 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00186692 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hurle Juan Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Polígono de Cazoña, E-39011, Santander, Spain aut NATIONALLICENCE 700 1- Colombatti Alfonso Divisone di Oncologia, Centro di Riferimento Oncologico, Sperimentale 2, I-33081, Aviano, Italy aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 355-364 0340-2061 193:4<355 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer