caa a22 4500 477095461 CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00186693 doi (NATIONALLICENCE)springer-10.1007/BF00186693 Characterization of Meibomian gland innervation in the cynomolgus monkey ( Macaca fascicularis ) [Elektronische Daten] [Wolfram Kirch, Markus Horneber, Ernst Tamm] To characterize the innervation of the cynomolgus monkey (Macaca fascicularis) Meibomian (tarsal) glands, upper lids of six cynomolgus monkeys were investigated with electronmicroscopical and double-labeling immunocytochemical methods. Antibodies against calcitonin gene-related peptide (CGRP), dopamine-β-hydroxylase (DBH), neuropeptide Y (NPY), nitric oxide synthase (NOS), protein gene product 9.5 (PGP 9.5), substance P (SP), tyrosine hydroxylase (TH), and vasoactive intestinal peptide (VIP) were used. In addition, sections were processed for NADPH-diaphorase (NADPH-d) histochemistry. Staining for PGP 9.5 and electron microscopy showed that Meibomian gland acini were surrounded by a network of unmyelinated nerves and terminal varicose axons. The terminals contained small agranular (30-60 nm) and large granular vesicles (65-110 nm), and were observed in close contact with the basal lamina of the acini, but never internally to the basal lamina. Meibomian axons showed like-immunore-activity (LI) for the neuropeptides SP, CGRP, NPY, and VIP. In addition, the axons stained for TH, DBH, NOS, and NADPH-d. VIP-LI, NOS- and NADPH-d-positive axons appeared to be more numerous, TH- and DBH-positive axons more rare than others. Most SP-LI axons were double-labelled for CGRP-LI, some for VIP-LI or NPY-LI. In addition, some VIP-LI axons were double-labeled for NPY-LI. NPY/VIP-LI and NPY/SP-LI axons were only observed close to the Meibomian acini. Conversely, NPY-LI colocalized with TH-IR or DBH-IR predominated in perivascular nerves of Meibomian gland vasculature. The close association of varicose axons with the acini of Meibomian glands indicates that nervous signals modulate meibomian secretion. Meibomian gland nerve fibers in the cynomolgus monkey appear to utilize various neuropeptides, catecholamines and nitric oxide as transmitter substances, and seem to derive from the pterygopalatine, superior cervical and trigeminal ganglion respectively. Springer-Verlag, 1996 Neuropeptide nationallicence Nitric oxide nationallicence Eyelid nationallicence Double-labeling method nationallicence Immunocytochemistry nationallicence Kirch Wolfram Department of Anatomy II, University of Erlangen-Nürnberg, Universitätsstrasse 19, D-91054, Erlangen, Germany aut Horneber Markus Department of Anatomy II, University of Erlangen-Nürnberg, Universitätsstrasse 19, D-91054, Erlangen, Germany aut Tamm Ernst National Eye Institute, Laboratory of Molecular and Developmental Biology, 6 Center Drive, MSC 2730, Building 6/Room 204, 20892, Bethesda, MD, USA aut Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 365-375 0340-2061 193:4<365 1996 193 429 https://doi.org/10.1007/BF00186693 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00186693 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirch Wolfram Department of Anatomy II, University of Erlangen-Nürnberg, Universitätsstrasse 19, D-91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Horneber Markus Department of Anatomy II, University of Erlangen-Nürnberg, Universitätsstrasse 19, D-91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Tamm Ernst National Eye Institute, Laboratory of Molecular and Developmental Biology, 6 Center Drive, MSC 2730, Building 6/Room 204, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 365-375 0340-2061 193:4<365 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer