caa a22 4500 47709550X CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00186698 doi (NATIONALLICENCE)springer-10.1007/BF00186698 The organization of microtubules and filamentous actin in cytospin preparations of Sertoli cells from w/w Wei mutant mice devoid of germ cells [Elektronische Daten] [Klaus Werner Wolf, Heinz Winking] The microtubule (MT) cytoskeleton and the distribution of filamentous actin (F-actin) were examined in the w/w Wei mutant of the house mouse (Mus musculus), which is characterized by the absence of germ cells. Cytospin preparations of carefully minced testis tubules were stained with one of three different monoclonal antitubulin antibodies or with phalloidin conjugated with a fluorescent dye. The antibodies were directed to β-tubulin, acetylated α-tubulin, and tyrosinated α-tubulin. The antibody to β-tubulin revealed prominent MT bundles oriented parallel to the long axes of the Sertoli cells. Acetylated α-tubulin was present only in a small subset of the MTs close to the nuclei, and tyrosinated α-tubulin was virtually missing in polymerized MTs. This pattern does not correspond to that reported for Sertoli cells in wild mammals. Thus, the absence of germ cells apparently affects the microtubular cytoskeleton. Our study showed that this also applies to F-actin. Bundles of F-actin were found throughout about two thirds of the Sertoli cellscytoplasm lateral to the nucleus in w/w Wei mice. In contrast, F-actin was reported to form sheets throughout the cytoplasm in wild-type mice. F-actin is part of the so-called ectoplasmic specializations — elements that are involved in the contact between the spermatids and Sertoli cells. Thus, the absence of germ cells from testes appears to have far-reaching effects on the cytoskeleton of the Sertoli cells. Springer-Verlag, 1996 Cytoskeleton nationallicence Ectoplasmic specializations nationallicence Mitosis nationallicence Tubulin nationallicence acet-MT : Acetylated microtubule nationallicence BSA : bovine serum albumin nationallicence DAPI : 4′,6-diamidino-2-phenylindole-2 HCl nationallicence EC : ectoplasmic specialization nationallicence EGTA : ethylene glycol-bis (β-aminoethyl ether)-N,N′-tetraacetic acid nationallicence F-actin : filamentous actin nationallicence FITC : fluorescein isothyocyanate nationallicence PBS : phosphate-buffered saline nationallicence PIPES : piperazine-N,N′ bis(2-ethane sulfonic acid) nationallicence MT : microtubule nationallicence tyr-MT : tyrosinated microtubule nationallicence Werner Wolf Klaus Institut für Biologie der Medizinischen Universität Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany aut Winking Heinz Institut für Biologie der Medizinischen Universität Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany aut Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 413-417 0340-2061 193:4<413 1996 193 429 https://doi.org/10.1007/BF00186698 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00186698 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Werner Wolf Klaus Institut für Biologie der Medizinischen Universität Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Winking Heinz Institut für Biologie der Medizinischen Universität Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/4(1996-04-01), 413-417 0340-2061 193:4<413 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer