caa a22 4500 477095569 CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00185876 doi (NATIONALLICENCE)springer-10.1007/BF00185876 Characterization of chondrogenesis in cells isolated from limb buds in mouse [Elektronische Daten] [Caroline Edwall-Arvidsson, Joanna Wroblewski] Micromass cultures of mesenchymal cells isolated from limb buds of 11.5-day-old mouse fetuses were used to study chondrogenesis. After 3 days of culture, dense cell aggregates were observed. They then were converted into macroscopically visible cartilage foci during the following 2-4 days. Comparison of 2-, 4- and 7-day-old cultures has shown that the cells first expressed collagen type I, then switched to collagen type II expression as shown by immunohistochemistry and in situ hybridization. At day 7, proteoglycans were synthesized centrally in the foci. At the same time, most cells expressed collagen type II, with the highest expression in the periphery of the aggregates. The oncogene c-fos and homeodomain protein FS-1 were found in the cells expressing collagen type II, indicating that these transcription factors may be involved in the regulation of cell differentiation. The expression of alkaline phosphatase was detected first in mature cartilage foci (day 4) and increased during culture. Early in culture, DNA-replicating cells were uniformly distributed. With differentiation, the proliferating cells were present predominantly between the aggregates and their total number became significantly reduced. Our results indicate that the process of chondrogenesis in micromass cultures of mesenchymal cells mimics the differentiation process occurring during fetal development in vivo and can be directly studied by in situ hybridization, immunohistochemical and histochemical methods. Springer-Verlag, 1996 Cartilage nationallicence Differentiation nationallicence DNA synthesis nationallicence Collagens nationallicence c-fos nationallicence Edwall-Arvidsson Caroline Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, S-171 77, Stockholm, Sweden aut Wroblewski Joanna Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, S-171 77, Stockholm, Sweden aut Anatomy and Embryology Springer-Verlag 193/5(1996-05-01), 453-461 0340-2061 193:5<453 1996 193 429 https://doi.org/10.1007/BF00185876 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185876 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edwall-Arvidsson Caroline Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 700 1- Wroblewski Joanna Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/5(1996-05-01), 453-461 0340-2061 193:5<453 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer