caa a22 4500 47709564X CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00214702 doi (NATIONALLICENCE)springer-10.1007/BF00214702 Immunohistochemical localization of estrogen receptors within aromatase-immunoreactive neurons in the fetal and neonatal rat brain [Elektronische Daten] [Yoshihiro Tsuruo, Kazunori Ishimura, Shinji Hayashi, Yoshio Osawa] We elucidated the anatomical relationship between estrogen receptors and aromatase, the enzyme converting androgens to estrogens, in the fetal and neonatal rat brain by means of double immunohistochemical labeling, using antibodies against rat estrogen receptors and human placental aromatase cytochrome P450. Numerous aromatase-immunoreactive neurons were found in the medial preoptic area, the bed nucleus of the stria terminalis, the medial amygdaloid nucleus and the ventromedial nucleus. Estrogen receptors were also abundant in these areas. Most of the aromatase-immunoreactive neurons showed immunoreactivity for estrogen receptors in the medial subdivision of the bed nucleus of the stria terminalis and in the posterodorsal division of the medial amygdaloid nucleus. There were also many double-labeled cells in the ventromedial nucleus. However, in the medial preoptic area the localization of aromatase-immunoreactive neurons was distinct from that of neurons containing estrogen receptors. These results suggested that estrogens, which are converted from androgens in aromatase-containing neurons, are involved in the sexual differentiation of the brain through estrogen receptors within aromatase-immunoreactive neurons in the bed nucleus of the stria terminalis, the medial amygdaloid nucleus and the ventromedial nucleus, but through estrogen receptors in aromatase-immunonegative neurons in the medial preoptic area. Springer-Verlag, 1996 Aromatase nationallicence Estrogen receptor nationallicence Immunohistochemistry nationallicence Brain nationallicence Rat nationallicence Tsuruo Yoshihiro Department of Anatomy, School of Medicine, The University of Tokushima, 770, Tokushima, Japan aut Ishimura Kazunori Department of Anatomy, School of Medicine, The University of Tokushima, 770, Tokushima, Japan aut Hayashi Shinji Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neuroscience, 183, Tokyo, Japan aut Osawa Yoshio Endocrine Biochemistry Department, Hauptman-Woodward Medical Research Institute, 14203, Buffalo, NY, USA aut Anatomy and Embryology Springer-Verlag 193/2(1996-02-01), 115-121 0340-2061 193:2<115 1996 193 429 https://doi.org/10.1007/BF00214702 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00214702 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsuruo Yoshihiro Department of Anatomy, School of Medicine, The University of Tokushima, 770, Tokushima, Japan aut NATIONALLICENCE 700 1- Ishimura Kazunori Department of Anatomy, School of Medicine, The University of Tokushima, 770, Tokushima, Japan aut NATIONALLICENCE 700 1- Hayashi Shinji Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neuroscience, 183, Tokyo, Japan aut NATIONALLICENCE 700 1- Osawa Yoshio Endocrine Biochemistry Department, Hauptman-Woodward Medical Research Institute, 14203, Buffalo, NY, USA aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/2(1996-02-01), 115-121 0340-2061 193:2<115 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer