caa a22 4500 477095658 CHVBK 20180405111526.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00214703 doi (NATIONALLICENCE)springer-10.1007/BF00214703 Relationship between altered axial curvature and neural tube closure in normal and mutant ( curly tail ) mouse embryos [Elektronische Daten] [Marian Peeters, Johan Hekking, Henny van Straaten, Alisa Shum, Andrew Copp] Neural tube defects, including spina bifida, develop in the curly tail mutant mouse as a result of delayed closure of the posterior neuropore at 10.5 days of gestation. Affected embryos are characterized by increased ventral curvature of the caudal region. To determine whether closure of the neuropore could be affected by this angle of curvature, we experimentally enhanced the curvature of non-mutant embryos. The amnion was opened in 9.5 day embryos; after 20 h of culture, a proportion of the embryos exhibited a tightly wrapped amnion with enhanced curvature of the caudal region compared with the control embryos in which the opened amnion remained inflated. Enhanced curvature correlated with a higher frequency of embryos with an open posterior neuropore, irrespective of developmental stage within the range, 27-32 somites. Thus, within this somite range, caudal curvature is a more accurate determinant for normal spinal neurulation than the exact somite stage. Enhanced ventral curvature of the curly tail embryo correlates with an abnormal growth difference between the neuroepithelium and ventral structures (the notochord and hindgut). We experimentally corrected this imbalance by culturing under conditions of mild hyperthermia and subsequently determined whether the angle of curvature would also be corrected. The mean angle of curvature and length of the posterior neuropore were both reduced in embryos cultured at 40.5°C by comparison with control embryos cultured at 38°C. We conclude that the sequence of morphogenetic events leading to spinal neural tube defects in curly tail embryos involves an imbalance of growth rates, which leads to enhanced ventral curvature that, in turn, leads to delayed closure of the posterior neuropore. Springer-Verlag, 1996 Curly tail mutant nationallicence Mouse nationallicence Neural tube defects nationallicence Spina bifida nationallicence Peeters Marian Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut Hekking Johan Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut van Straaten Henny Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut Shum Alisa Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut Copp Andrew Division of Cell and Molecular Biology, Institute of Child Health, University of London, 30 Guilford Street, WC1N 1EH, London, UK aut Anatomy and Embryology Springer-Verlag 193/2(1996-02-01), 123-130 0340-2061 193:2<123 1996 193 429 https://doi.org/10.1007/BF00214703 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00214703 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peeters Marian Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut NATIONALLICENCE 700 1- Hekking Johan Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut NATIONALLICENCE 700 1- van Straaten Henny Department of Anatomy and Embryology, Faculty of Medicine, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, Netherlands aut NATIONALLICENCE 700 1- Shum Alisa Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut NATIONALLICENCE 700 1- Copp Andrew Division of Cell and Molecular Biology, Institute of Child Health, University of London, 30 Guilford Street, WC1N 1EH, London, UK aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 193/2(1996-02-01), 123-130 0340-2061 193:2<123 1996 193 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer