caa a22 4500 477095917 CHVBK 20180405111527.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00187135 doi (NATIONALLICENCE)springer-10.1007/BF00187135 Chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of rat tibial articular cartilage [Elektronische Daten] [Yasuyuki Sasano, Mitsuru Furusawa, Haruo Ohtani, Itaru Mizoguchi, Ichiro Takahashi, Manabu Kagayama] The present study was designed to investigate whether or not chondrocytes in articular cartilage express type I collagen in vivo under physiological conditions. Expressions of the gene and the phenotype of type I collagen were examined in rat tibial articular cartilage in the knee joint during development. Knee joints of Wistar rats at 1, 5, and 11 weeks postnatal were fixed in 4% paraformaldehyde with or without 0.5% glutaraldehyde and decalcified in 10% EDTA. After the specimens were embedded in paraffin and serial sections made, adjacent sections were processed for immunohistochemistry and in situ hybridization for type I collagen. The epiphysis of the tibia was composed of cartilage in week-1 rats. Formation of articular cartilage was in progress in week 5 as endochondral ossification proceeded and was completed in week 11. Anti-type I collagen antibody stained only the superficial area of the epiphysis in week 1, but the immunoreactivity was expanded into the deeper region of the articular cartilage with development in weeks 5 and 11. Hybridization signals for pro-alpha 1 (I) collagen were seen in some of chondrocytes in the epiphysis of the week-1 tibia. The most intense signals were identified in chondrocytes in week 5 and the signals appeared weaker in week 11. The present study demonstrated that chondrocytes synthesize type I collagen and accumulate the protein in the matrix during development of the articular cartilage. Springer-Verlag, 1996 Articular cartilage nationallicence Development nationallicence Type I collagen nationallicence In situ hybridization nationallicence Immunohistochemistry nationallicence Sasano Yasuyuki Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut Furusawa Mitsuru Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut Ohtani Haruo Department of Pathology, Tohoku University School of Medicine, 980-77, Sendai, Japan aut Mizoguchi Itaru Department of Orthodontics, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut Takahashi Ichiro Department of Orthodontics, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut Kagayama Manabu Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 247-252 0340-2061 194:3<247 1996 194 429 https://doi.org/10.1007/BF00187135 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187135 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasano Yasuyuki Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut NATIONALLICENCE 700 1- Furusawa Mitsuru Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut NATIONALLICENCE 700 1- Ohtani Haruo Department of Pathology, Tohoku University School of Medicine, 980-77, Sendai, Japan aut NATIONALLICENCE 700 1- Mizoguchi Itaru Department of Orthodontics, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut NATIONALLICENCE 700 1- Takahashi Ichiro Department of Orthodontics, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut NATIONALLICENCE 700 1- Kagayama Manabu Second Department of Oral Anatomy, Tohoku University School of Dentistry, 980-77, Sendai, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 247-252 0340-2061 194:3<247 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer