caa a22 4500 47709595X CHVBK 20180405111527.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00187136 doi (NATIONALLICENCE)springer-10.1007/BF00187136 Epidermal growth factor receptor in adult human dorsal root ganglia [Elektronische Daten] [J. Huerta, R. Diaz-Trelles, F. Naves, M. Llamosas, M. Del Valle, J. Vega] Transforming growth factor-α (TGFα) enhances neuronal survival and neurite outgrowth in cultured dorsal root ganglia (DRG) sensory neurons. It binds a membrane protein, denominated epidermal growth factor receptor (EGFr). EGFr has been localized in developing and adult human DRG. However, it remains to be elucidated whether all DRG neurons express EGFr or whether differences exist among neuronal subtypes. This study was undertaken to investigate these topics in adult human DRG using immunoblotting, and combined immunohistochemistry and image analysis techniques. A mouse monoclonal antibody (clone F4) mapping within the intracytoplasmic domain of EGFr was used. Immunoblotting revealed two main proteins with estimated molecular masses of ∼- 65 kDa and 170 kDa, and thus consistent with the full-length EGFr. Additional protein bands were also encountered. Light immunohistochemistry revealed specific immunoreactivity (IR) for EGFr-like proteins in most (86%) primary sensory neurons, the intensity of immunostaining being stronger in the small- and intermediate-sized ones. Furthermore, EGFr-like IR was also observed in the satellite glial cells of the ganglia as well as in the intraganglionic and dorsal root Schwann cells. Taken together, our findings demonstrate that EGFr, and other related proteins containing the epitope labeled with the antibody F4, are responsible for the EGFr IR reported in DRG. Furthermore, we demonstrated heterogeneity in the expression of EGFr-like IR in adult human primary sensory neurons, which suggests different responsiveness to their ligands. Springer-Verlag, 1996 Epidermal growth factor receptor nationallicence Dorsal root ganglia nationallicence Immunoblotting nationallicence Immunohistochemistry nationallicence Human nationallicence Huerta J. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut Diaz-Trelles R. Departamento de Biologia Funcional, Universidad de Oviedo, E-3006, Oviedo, Spain aut Naves F. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut Llamosas M. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut Del Valle M. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut Vega J. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 253-257 0340-2061 194:3<253 1996 194 429 https://doi.org/10.1007/BF00187136 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187136 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huerta J. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 700 1- Diaz-Trelles R. Departamento de Biologia Funcional, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 700 1- Naves F. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 700 1- Llamosas M. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 700 1- Del Valle M. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 700 1- Vega J. Departamento de Morfología y Biología Celular, Universidad de Oviedo, E-3006, Oviedo, Spain aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 253-257 0340-2061 194:3<253 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer