caa a22 4500 47709600X CHVBK 20180405111527.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00187133 doi (NATIONALLICENCE)springer-10.1007/BF00187133 Yuasa Shigeki Laboratory of Neural Information, National Institute for Physiological Sciences, 444, Okazaki, Japan aut Bergmann glial development in the mouse cerebellum as revealed by tenascin expression [Elektronische Daten] [Shigeki Yuasa] Tenascin, an astroglia-derived extracellular matrix molecule, is also expressed by radial glia of the embryonic mouse cerebellum. Expression of tenascin can thus be applied as a marker of astroglial development from an early stage, especially prior to the expression of the glial fibrillary acidic protein (GFAP) that can be detected in the postnatal cerebellum. The development of Bergmann glia, specialized cerebello-cortical astroglia with radial processes, was examined by tenascin immunohistochemistry and non-radioactive in situ hybridization histochemistry for tenascin mRNA in the developing mouse cerebellum. Tenascin-immunopositive radial glial processes extending from the ventricular zone to the pia mater retracted toward the cortex in the embryonic cerebellum and occupied a position corresponding to the Bergmann glial processes at the perinatal stage. Tenascin gene-expressing cells were generated in the ventricular zone of the cerbellar primordium and migrated radially toward the cortex. They were stratified in the layer of Bergmann glial somata at the early postnatal stage. They extended GFAP-immunopositive radial processes from the somata to the pia mater as revealed by double-labeling employing tenascin in situ hybridization histochemistry and GFAP-immunostaining. Bergmann glia are therefore considered to develop from cerebellar radial glia by migration of their somata and retraction of their processes. The tenascin gene-expressing cells displayed mitotic activity after alignment in the cortex as revealed by double-labeling by tenascin in situ hybridization histochemistry and immunohistochemical detection of the incorporated bromodeoxyuridine. The above findings suggest that the Bergmann glia in the cortex represent one of the origins of the astroglia in the developing cerebellum. Springer-Verlag, 1996 In situ hybridization histochemistry nationallicence Radial glia nationallicence Astroglia nationallicence GFAP nationallicence Cell migration nationallicence Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 223-234 0340-2061 194:3<223 1996 194 429 https://doi.org/10.1007/BF00187133 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00187133 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Yuasa Shigeki Laboratory of Neural Information, National Institute for Physiological Sciences, 444, Okazaki, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/3(1996-09-01), 223-234 0340-2061 194:3<223 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer