caa a22 4500 477096026 CHVBK 20180405111527.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00198536 doi (NATIONALLICENCE)springer-10.1007/BF00198536 Distribution of immunoreactive 2′,5′-oligoadenylate synthetase in mouse reproductive organs [Elektronische Daten] [Mari Asada-Kubota, Mikiko Kobayashi, Tetsuo Ueda, Mamoru Shimada, Kazumasa Takeda, Kazushige Hamada, Shohei Maekawa, Yoshihiro Sokawa] Although 2′,5′-oligoadenylate synthetase (25AS) is an enzyme induced by inferferon (IFN) or viral infections and mediates one of the principal antiviral pathways turned on by IFN, low constitutive levels of the enzyme can be detected in various "normal” animals that have not been treated with IFN or virus. The distribution of this enzyme in the female and male reproductive organs of normal healthy mice was studied by Western blotting and by an immunohistochemical method, using a specific monoclonal antibody. On Western blotting, an antibody to 42-kD 2-5AS reacted with extracts from the ovary, oviduct, uterus, vagina, and placenta among the female reproductive organs, and testis, epididymis, and ductus deferens in the male. Immunohistochemically, the 2-5AS was localized on the following cells in the female reproductive organs: oocytes in the ovary; epithelium in the oviduct, uterus, and vagina; and trophoblasts in the placenta. Furthermore, the 2-5AS was localized on the epithelium and muscular layer in the ductus deferens and epithelium in the penis of the male mice, whereas the epithelium of the testis, epididymis, and seminal vesicle were stained faintly. It is well known that IFN is produced continuously in normal mice, so the 2-5AS in the tissues of normal mice is considered to be induced by such IFN produced under physiological conditions. Expression of the 2-5AS on the epithelium and trophoblasts in the reproductive organs may be responsible for the prevention of viral infections. However, the enzyme in oocytes may have some functions other than as an antiviral agent, since the enzyme was not detectable in embryos during early development. Springer-Verlag, 1996 2′,5′-Oligoadenylate synthetase nationallicence Immunohistochemistry nationallicence Mouse nationallicence Reproductive organs nationallicence Oocyte nationallicence Asada-Kubota Mari Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Kobayashi Mikiko Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Ueda Tetsuo Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Shimada Mamoru Cell Science Laboratory, Research Center, Nippon Paint Co. LTD., 572, Osaka, Japan aut Takeda Kazumasa Institute for Virus Research, Kyoto University, 606, Kyoto, Japan aut Hamada Kazushige Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Maekawa Shohei Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Sokawa Yoshihiro Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 349-354 0340-2061 194:4<349 1996 194 429 https://doi.org/10.1007/BF00198536 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198536 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asada-Kubota Mari Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Kobayashi Mikiko Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Ueda Tetsuo Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Shimada Mamoru Cell Science Laboratory, Research Center, Nippon Paint Co. LTD., 572, Osaka, Japan aut NATIONALLICENCE 700 1- Takeda Kazumasa Institute for Virus Research, Kyoto University, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Hamada Kazushige Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Maekawa Shohei Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Sokawa Yoshihiro Department of Biotechnology, Kyoto Institute of Technology, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 349-354 0340-2061 194:4<349 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer