caa a22 4500 477096042 CHVBK 20180405111527.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00198543 doi (NATIONALLICENCE)springer-10.1007/BF00198543 Immunolocalization of peptide 19 and other calcium-binding proteins in the guinea pig cochlea [Elektronische Daten] [Shun-Ichi Imamura, Joe Adams] Calcium ions are known to play critical roles in a variety of cochlear functions. The distributions of a number of calcium binding proteins that regulate calcium ion levels within the cochlea have previously been described. In this report we extend and refine previous reports of the distribution of immunostaining for calmodulin, calbindin, and calretinin and show for the first time the distribution for peptide 19. There were longitudinal and radial gradients of immunostaining for peptide 19 within outer hair cells that appeared to match previously described gradients of efferent innervation of these cells. Gradients of immunostaining for calbindin within outer hair cells were in the opposite directions, which suggests that levels of this protein are correlated with afferent innervation density and perhaps the abundance of subsurface cisternae. No gradients were seen in the distributions of cells stained for calmodulin and calretinin, which included sensory cells and supporting cells respectively. All ganglion cells were stained for calmodulin but the other proteins appeared to be present in limited ganglion cell subpopulations. In addition to staining of sensorineural elements, antisera to all compounds but peptide 19 showed immunostaining of cells within the lateral wall and the spiral limbus. The results suggest that the proteins under study are involved in a wide variety of calcium-regulated functions within the cochlea. Knowledge of the unique distribution of each of the compounds should facilitate further studies of their roles in cochlear function. Springer-Verlag, 1996 Calbindin nationallicence Calretinin nationallicence Calmodulin nationallicence Organ of Corti nationallicence Spiral ganglion nationallicence Imamura Shun-Ichi Department of Otolaryngology, Yamanashi Medical University, Yamanashi, Japan aut Adams Joe Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 243 Charles Street, 02114, Boston, MA, USA aut Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 407-418 0340-2061 194:4<407 1996 194 429 https://doi.org/10.1007/BF00198543 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198543 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Imamura Shun-Ichi Department of Otolaryngology, Yamanashi Medical University, Yamanashi, Japan aut NATIONALLICENCE 700 1- Adams Joe Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 243 Charles Street, 02114, Boston, MA, USA aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 407-418 0340-2061 194:4<407 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer