caa a22 4500 477096069 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00198539 doi (NATIONALLICENCE)springer-10.1007/BF00198539 Calretinin immunoreactivity in human sympathetic ganglia [Elektronische Daten] [J. Huerta, S. Nori, M. Llamosas, M. Vázquez, E. Bronzetti, J. Vega] Calretinin is an "EF-hand” calcium-binding protein involved in the maintenance of intracellular calcium ion homeostasis. This study was understaken to investigate the presence of calretinin in human lumbar paravertebral sympathetic ganglia from subjects of different ages (26-85 years) using immunohistochemical and immunoblotting methods. Calretinin-like immunoreactivity was found in a subpopulation of postganglionic sympathetic neurons, whose percentage decreased progressively with aging by about 50% (63% of immunoreactive neurons at ≤40 years; 29% at ≥81 years) whereas the neuronal density remained basically unchanged. Calretinin-like immunoreactivity showed a granular pattern of cytoplasmic distribution suggesting preferential localization of this protein associated with intracellular membranes. Occasionally diffuse cytosolic labelling was also observed. The immunoblotting demonstrated a protein band with an estimated molecular weight of 30 kDa, approximately. Present results provide, for the first time, evidence for the presence of calretinin in human paravertebral sympathetic ganglia. Since the number of calretinin-like immunoreactive neurons decreased significantly with aging our findings suggest an involvement of this protein in the age-dependent impairment of sympathetic function. Springer-Verlag, 1996 Paravertebral sympathetic ganglia nationallicence Calretinin nationallicence Aging nationallicence Immunoblotting nationallicence Immunohistochemistry nationallicence Human nationallicence Huerta J. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut Nori S. Istituto di Anatomia, Facoltà di Medicina, Università Cattolica del "Sacro Cuore”, Roma, Italy aut Llamosas M. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut Vázquez M. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut Bronzetti E. Dipartimento di Scienze Cardiovasculari e Respiratorie, Università "La Sapienza”, Roma, Italy aut Vega J. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 373-378 0340-2061 194:4<373 1996 194 429 https://doi.org/10.1007/BF00198539 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198539 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huerta J. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Nori S. Istituto di Anatomia, Facoltà di Medicina, Università Cattolica del "Sacro Cuore”, Roma, Italy aut NATIONALLICENCE 700 1- Llamosas M. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Vázquez M. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Bronzetti E. Dipartimento di Scienze Cardiovasculari e Respiratorie, Università "La Sapienza”, Roma, Italy aut NATIONALLICENCE 700 1- Vega J. Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, E-33006, Oviedo, Spain aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 373-378 0340-2061 194:4<373 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer