caa a22 4500 477096085 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00198535 doi (NATIONALLICENCE)springer-10.1007/BF00198535 The role of macrophages in clearing programmed cell death in the developing kidney [Elektronische Daten] [Victoria Camp, Paul Martin] The metanephric kidney develops from two tissue sources, the metanephric mesenchymal blastema and the ureteric bud epithelium. Following a complex interplay of inductive signals between these two tissues, small groups of metanephric mesenchymal cells aggregate and epithelialise to form young nephrons. As this is happening, significant numbers of cells in close proximity to the forming nephrons undergo programmed cell death or apoptosis. In this paper we investigate the clearance of developmental cell death in the mouse kidney between embryonic days 11.5 and 16.5; specifically, we address the issue of whether specialist macrophages or non-specialist neighbouring mesenchymal cells are responsible for phagocytosis and removal of dying cells. We show, using a monoclonal antibody F4/80 that specifically recognizes murine macrophages, that whenever and wherever there is cell death in the developing mesonephric or metanephric kidney there are also haemopoietically derived specialist macrophages. Moreover, in the mesonephros and from E14.5 in the metanephric kidney, we see large numbers of macrophages clearly swollen with phagocytosed apoptotic bodies. Double-labelling experiments using the DNA dye 7AAD to reveal condensed apoptotic nuclei and F4/80 to reveal macrophage plasma membranes show definitively that the majority of dying cells in the developing kidney are engulfed by macrophages. Springer-Verlag, 1996 Mouse embryo nationallicence Macrophages nationallicence Mesonephros nationallicence Metanephros nationallicence Apoptosis nationallicence Camp Victoria Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK aut Martin Paul Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK aut Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 341-348 0340-2061 194:4<341 1996 194 429 https://doi.org/10.1007/BF00198535 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198535 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Camp Victoria Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK aut NATIONALLICENCE 700 1- Martin Paul Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 341-348 0340-2061 194:4<341 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer