caa a22 4500 477096131 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00198538 doi (NATIONALLICENCE)springer-10.1007/BF00198538 Protein gene-product 9.5 in developing mouse circumvallate papilla: comparison with neuron-specific enolase and calcitonin gene-related peptide [Elektronische Daten] [S. Wakisaka, Y. Miyawaki, S. Youn, J. Kato, K. Kurisu] The present study was made to investigate the ontogeny of protein gene-product 9.5 (PGP 9.5)-like immunoreactivity (-LI) in the developing mouse circumvallate papilla (CVP), and its distribution was compared to that of neuron-specific enolase (NSE) and calcitonin gene-related peptide (CGRP). In adult CVP, PGP 9.5-LI was observed in the subgemmal nerve plexus; some thin PGP 9.5-like immunoreactive (-IR) nerve fibers penetrated taste buds and apical epithelium. PGP 9.5-LI was also observed in the spindle-shaped cells in taste buds, and a small number of round- or oval-shaped ganglionic cells in the lamina propria. The distribution of NSE-LI was comparable to that of PGP 9.5-LI. CGRP-LI was observed in the nerve fibers only; distribution of CGRP-IR nerve fibers was similar to that of PGP 9.5-IR nerve fibers, although the number of CGRP-IR nerve fibers was smaller than that of PGP 9.5-IR nerve fibers. At least six developmental stages were defined with regard to the developmental changes in the distribution of PGP 9.5-LI from embryonic day (E) 12 to adulthood: Stage I (E12-13) — a dense nerve plexus of PGP 9.5-IR nerve fibers was detected in the lamina propria beneath the core of newly-formed papilla. Stage II (E14-16) — thin PGP 9.5-IR nerve fibers penetrated the apical epithelium, and a few round-shaped cells in the apical epithelium also displayed PGP 9.5-LI. Stage III (E17-18) — thin PGP 9.5-IR nerve fibers penetrated the inner lateral epithelium of the trench. Stage IV [Postnatal day (P) 0-3] many PGP 9.5-IR nerve fibers penetrated the outer lateral epithelium of the trench; later in this stage, taste buds appeared. Stage V (P5-10) — a small number of PGP 9.5 IR cells in the taste buds appeared, and their number increased gradually. Stage VI (PI4-adult) — the number of PGP 9.5-IR taste cells increased and reached the adult level, while the number of PGP 9.5-IR nerve fibers decreased. The development of NSE-LI was similar to that of PGP 9.5-LI. CGRP-IR nerve fibers were detected at E12 in the lamina propria, and the development of the intraepithelial CGRP-IR nerve fibers was similar to that of PGP 9.5-IR nerve fibers. The present results indicate that invasion by nerve fibers of the epithelium of lingual papillae occurs in a complex manner, and that these nerve fibers may participate in the formation of the taste buds. Springer-Verlag, 1996 Ontogeny nationallicence Circumvallate papilla nationallicence Taste buds nationallicence Innervation nationallicence Protein gene-product 9.5 nationallicence Neuron-specific enolase nationallicence Calcitonin gene-related peptide nationallicence Immunohistochemistry nationallicence Mouse nationallicence Wakisaka S. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut Miyawaki Y. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut Youn S. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut Kato J. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut Kurisu K. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 365-372 0340-2061 194:4<365 1996 194 429 https://doi.org/10.1007/BF00198538 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00198538 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wakisaka S. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Miyawaki Y. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Youn S. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Kato J. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Kurisu K. Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/4(1996-10-01), 365-372 0340-2061 194:4<365 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer