caa a22 4500 477096158 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00185992 doi (NATIONALLICENCE)springer-10.1007/BF00185992 Isolation and characterization of endothelial cells from different organs of fetal pigs [Elektronische Daten] [Johanna Plendl, Christine Neumüller, Angelika Vollmar, Robert Auerbach, Fred Sinowatz] The purpose of this study was to isolate endothelial cells from different organs of porcine fetuses and to examine the binding of endothelial markers including lectins. Endothelial cells were isolated from the aorta, cerebral cortex, myocardium, ovary and testis. Binding of the antibodies to von Willebrand factor (vWF) and angiotensin converting enzyme (ACE), the presence of Weibel Palade bodies (WPB), uptake of acetylated low density lipoprotein (acLDL), and labelling with the lectins Bandeiraea simplicifolia agglutinin I (BS I), Peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA) and Ulex europaeus agglutinin I (UEA I) were examined. Cell preparations displayed cobblestone-like morphology with the exception of testicular endothelium, which formed arcuate structures. Endothelium isolated from the brain labelled more strongly than any other cell line with the lectin PNA, but it did not express ACE. In contrast to other cell preparations, myocardial endothelium showed very low binding of anti-vWF. Ovarian endothelium was able to perform in vitro angiogenesis. Moreover, these endothelial cells possessed the largest number of WPB. Testicular endothelium displayed highest binding of vWF. Endothelium isolated from the aorta, in contrast to all other endothelial cells, did not take up acLDL. These results demonstrate that organ- and tissue-specific heterogeneity is already expressed in fetal endothelium. Springer-Verlag, 1996 Endothelium nationallicence Fetus nationallicence Porcine nationallicence In vitro nationallicence Lectins nationallicence Plendl Johanna University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut Neumüller Christine University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut Vollmar Angelika University of Munich, Faculty of Veterinary Medicine, Institute of Toxicology and Pharmacology, Veterinärstrasse 13, D-80539, Munich, Germany aut Auerbach Robert Zoology Research, Center for Developmental Biology, 1117 West Johnson Street, WI 53706, Madison, USA aut Sinowatz Fred University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut Anatomy and Embryology Springer-Verlag 194/5(1996-11-01), 445-456 0340-2061 194:5<445 1996 194 429 https://doi.org/10.1007/BF00185992 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185992 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Plendl Johanna University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut NATIONALLICENCE 700 1- Neumüller Christine University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut NATIONALLICENCE 700 1- Vollmar Angelika University of Munich, Faculty of Veterinary Medicine, Institute of Toxicology and Pharmacology, Veterinärstrasse 13, D-80539, Munich, Germany aut NATIONALLICENCE 700 1- Auerbach Robert Zoology Research, Center for Developmental Biology, 1117 West Johnson Street, WI 53706, Madison, USA aut NATIONALLICENCE 700 1- Sinowatz Fred University of Munich, Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Veterinärstrasse 13, D-80539, Munich, Germany aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/5(1996-11-01), 445-456 0340-2061 194:5<445 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer