caa a22 4500 477096212 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00185996 doi (NATIONALLICENCE)springer-10.1007/BF00185996 Tectorial membrane-organ of Corti relationship during cochlear development [Elektronische Daten] [Joaquin Rueda, Raquel Cantos, David Lim] The development of stereociliary attachment to the tectorial membrane was investigated in the mouse cochlea using transmission and scanning electron microscopy. At the 18th gestational day, only the major tectorial membrane can be identified covering the greater epithelial ridge and the inner hair cells in all turns. At the 19th gestational day, the minor tectorial membrane was first seen in the basal turn, over the outer hair cells. During early stages of development, the stereocilia of hair cells were surrounded by a loose fibrillar material underneath the tectorial membrane. After the 10th postnatal day, the outer hair cells' stereocilia were attached to Kimura's (or Hardesty's) membrane, while inner hair cells' stereociliary bundles were attached to the undersurface of the tectorial membrane near the Hensen's stripe. Between the 10th and the 14th postnatal days, the space between the inner hair cells and the first row of outer hair cells widened by virtue of the growth of the heads of pillar cells, and the inner hair cells' stereocilia were displaced towards the Hensen's stripe. After the 14th postnatal day, the inner hair cells' stereociliary bundles detached from the tectorial membrane, while the outer hair cells' stereocilia remained attached to it. The tip-link system, which connects the tips of the stereocilia to the next tallest stereocilia, is present at birth in the outer hair cells. The marginal pillar, that anchored the tectorial membrane to the underlying organ of Corti during development, first appeared on the 6th postnatal day and disappeared on the 14th-15th postnatal day. The present data together with other reports support the idea that although some structures, such as hair cells' stereocilia and innervation, are already formed early during development, the cochlear microarchitecture is not fully developed morphologically and ready to function normally until the end of the second postnatal week in the mouse. Springer-Verlag, 1996 Inner ear nationallicence Hair cell nationallicence Stereociliary attachment nationallicence Mouse nationallicence Ultrastructure nationallicence Rueda Joaquin Departamento de Histologia, Facultad de Medicina, Universidad de Alicante, Apdo. Correos 374, E-03080, Alicante, Spain aut Cantos Raquel Departamento de Histologia, Facultad de Medicina, Universidad de Alicante, Apdo. Correos 374, E-03080, Alicante, Spain aut Lim David Laboratory of Cellular and Molecular Biology, House Ear Institute, Los Angeles, California, USA aut Anatomy and Embryology Springer-Verlag 194/5(1996-11-01), 501-514 0340-2061 194:5<501 1996 194 429 https://doi.org/10.1007/BF00185996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rueda Joaquin Departamento de Histologia, Facultad de Medicina, Universidad de Alicante, Apdo. Correos 374, E-03080, Alicante, Spain aut NATIONALLICENCE 700 1- Cantos Raquel Departamento de Histologia, Facultad de Medicina, Universidad de Alicante, Apdo. Correos 374, E-03080, Alicante, Spain aut NATIONALLICENCE 700 1- Lim David Laboratory of Cellular and Molecular Biology, House Ear Institute, Los Angeles, California, USA aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/5(1996-11-01), 501-514 0340-2061 194:5<501 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer