caa a22 4500 477096247 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195008 doi (NATIONALLICENCE)springer-10.1007/BF00195008 Cartilage canals in human thyroid cartilage characterized by immunolocalization of collagen types I, II, pro-III, IV and X [Elektronische Daten] [Horst Claassen, Thorsten Kirsch, Guido Simons] In this study the collagenous composition of cartilage canals in human thyroid cartilage, which are perichondral invaginations of blood vessels and connective tissue, and the surrounding cartilage matrix were investigated by immunolabelling with specific antibodies against type I, II, pro-III, IV and X collagen. During childhood and early adolescence no cartilage canals were detected in thyroid cartilage, and immunolabelling for type IV collagen was restricted to basal lamina components of blood vessels in the perichondrium. First immunolabelling for type IV collagen, belonging to blood vessels in cartilage canals, in both sexes was detected about the end of the second decade; it was localized in the dorsal part of the thyroid cartilage plate. At this time thyroid cartilage has already reached its final form and size. As revealed by von Kossa staining, vascularization preceded mineralization and ossification. In contrast to the male thyroid cartilage plate, no immunostaining for type IV collagen and no ossification was detected in the ventral half of female thyroid cartilage even in advanced age. The extracellular matrix of cells in cartilage canals showed positive immunostaining for collagen types I and pro-III as well as for collagen type II, indicating that the cells in the canal possess fibroblastic and chondrogenic properties. The extracellular matrix of hypertrophic chondrocytes adjacent to cartilage canals showed strong immunoreactivity for type X collagen. First mineralization was detected close to cartilage canals, suggesting that mineralization in human thyroid cartilage starts in the extracellular matrix adjacent to cartilage canals. Springer-Verlag, 1996 Thyroid cartilage nationallicence Immunohistochemistry nationallicence Vascularization nationallicence Cartilage canals nationallicence Collagens nationallicence Claassen Horst Anatomisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut Kirsch Thorsten Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, 4001 Spruce Street, 19104, Philadelphia, PA, USA aut Simons Guido Radiochemisches Laboratorium der Hoechst AG, Nieder Kirchweg/Ecke Stroofstrasse, D-65934, Frankfurt/M., Germany aut Anatomy and Embryology Springer-Verlag 194/2(1996-08-01), 147-153 0340-2061 194:2<147 1996 194 429 https://doi.org/10.1007/BF00195008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Claassen Horst Anatomisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut NATIONALLICENCE 700 1- Kirsch Thorsten Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, 4001 Spruce Street, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Simons Guido Radiochemisches Laboratorium der Hoechst AG, Nieder Kirchweg/Ecke Stroofstrasse, D-65934, Frankfurt/M., Germany aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/2(1996-08-01), 147-153 0340-2061 194:2<147 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer