caa a22 4500 477096263 CHVBK 20180405111528.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195007 doi (NATIONALLICENCE)springer-10.1007/BF00195007 Developmental analysis of GFAP immunoreactivity in the cerebellum of the meander tail mutant mouse [Elektronische Daten] [Holly Grishkat, Eric Schwartz, Gaurav Jain, Leonard Eisenman] It is thought that Bergmann glial fibers assist in the inward migration of granule cells. Model systems in which there is a perturbation of either the migrating cells or the glial cell population have been useful in understanding the migratory process. In the meander tail mutant mouse, the anterior cerebellar region is agranular, whereas the posterior cerebellum is relatively unaffected by the mutation. This study presents a qualitative analysis of the development of cerebellar radial glia in mea/mea and +/mea mice aged from postnatal day 0 to adult, using an antibody against the glia specific antigen, glial fibrillary acidic protein. The results indicate a slight delay in the onset of immunoreactivity in the mea/mea cerebellum and abnormal glial formation in the anterior and posterior regions by postnatal day 5. At postnatal day 11, the full complement of labeled fibers appears to be present and although they appear abnormal in formation, they eventually reach the surface and terminate in oddly shaped and irregularly spaced endfeet. In adult mea/mea and +/mea mice, as compared to the early postnatal stages, there is a significant reduction in GFAP immunoreactive fibers. Cresyl violet stained adult mea/mea sections revealed the presence of ectopic granule cells in radial columns and small clumps at the surface of and within the molecular layer of the caudal cerebellum. Quantitative analyses revealed a 4- to 5-fold increase in the number of ectopic granule cells in lobule VIII of the mea/mea when compared with the +/mea cerebellum. These results suggest that the radial glia in the mea/mea cerebellum exhibit some uncharacteristic morphologies, but that these abnormalities are most likely the consequence of environmental alterations produced by the mutant gene. Springer-Verlag, 1996 Glial fibrillary acidic protein nationallicence Bergmann glial fibers nationallicence Granule cells nationallicence Cerebellar mutation nationallicence Purkinje cell nationallicence Grishkat Holly Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut Schwartz Eric Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut Jain Gaurav Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut Eisenman Leonard Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut Anatomy and Embryology Springer-Verlag 194/2(1996-08-01), 135-146 0340-2061 194:2<135 1996 194 429 https://doi.org/10.1007/BF00195007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grishkat Holly Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Schwartz Eric Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Jain Gaurav Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Eisenman Leonard Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 773 0- Anatomy and Embryology Springer-Verlag 194/2(1996-08-01), 135-146 0340-2061 194:2<135 1996 194 429 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer