caa a22 4500 477099882 CHVBK 20180405111537.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196653 doi (NATIONALLICENCE)springer-10.1007/BF00196653 Characterization of three heat-shock-protein genes and their developmental regulation during somatic embryogenesis in white spruce [ Picea glauca (Moench) Voss] [Elektronische Daten] [Jin-Zhuo Dong, David Dunstan] Three cDNAs (PgEMB22, 27 and 29) predicted to encode low-molecular-weight (LMW) heat-shock proteins (HSPs) were cloned and characterized from white spruce [Picea glauca (Moench) Voss] somatic embryo tissues by differentially screening a cotyledonary embryo cDNA library. Clone PgEMB22 is predicted to encode a putative mitochondria-localized LMW HSP, and PgEMB27 and 29 are predicted to encode different cytoplasmic class II LMW HSPs, although they share 84.7% identity within DNA coding regions and 83.0% identity for predicted proteins. They are developmentally regulated during somatic embryo development and subsequent embryo germination, in addition they show strong response to heat-shock stress. Transcripts of the two kinds of hsp genes could be detected in embryogenic tissues before induction of embryo maturation, but subsequently increased, being most abundant at late embryo stages. Gene expression levels were very low or not detectable in germinated plantlets or needle tissues from older plants. Abscisic acid and polyethylene glycol, stimulators for spruce embryo maturation, could also induce the hsp genes. Springer-Verlag, 1996 Abscisic acid nationallicence Somatic embryogenesis nationallicence Gene expression nationallicence Heat shock protein nationallicence Picea nationallicence Polyethylene glycol nationallicence aa : amino acid nationallicence ABA : abscisic acid nationallicence HSP : heat-shock protein nationallicence Leu : leucine nationallicence LMW : low molecular weight nationallicence Met : methionine nationallicence ORF : open reading frame nationallicence PEG : polyethylene glycol nationallicence Dong Jin-Zhuo Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut Dunstan David Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut Planta Springer-Verlag 200/1(1996-09-01), 85-91 0032-0935 200:1<85 1996 200 425 https://doi.org/10.1007/BF00196653 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196653 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Jin-Zhuo Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Dunstan David Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 85-91 0032-0935 200:1<85 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer