caa a22 4500 477099890 CHVBK 20180405111537.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196655 doi (NATIONALLICENCE)springer-10.1007/BF00196655 New evidence for a role for thioredoxin h in germination and seedling development [Elektronische Daten] [Rosa Lozano, Joshua Wong, Boihon Yee, Anne Peters, Karoly Kobrehel, Bob Buchanan] Thioredoxin of the h-type — earlier linked to the reduction of wheat (Triticum durum Desf. cv. Monroe) endosperm proteins — was converted from an oxidized to a partially reduced state during germination and seedling development. While the abundance of thioredoxin progressively decreased during this period, the availability of reducing equivalents, defined as the product of the relative abundance of thioredoxin and the percent reduction, increased. The amount of the enzyme catalyzing the reduction of thioredoxin h (NADP-thioredoxin reductase) remained constant. The activities of enzymes generating the NADPH needed for the reduction of thioredoxin (glucose 6-phosphate and 6-phosphogluconate dehydrogenases) increased. The level of thioredoxin h in the endosperm appeared to be controlled by the embryo via hormones. Gibberellic acid enhanced the disappearance of thioredoxin, whereas abscisic acid showed the opposite effect. Moreover, uniconazole, an inhibitor of gibberellic acid synthesis, slowed seedling growth and inhibited the disappearance of thioredoxin in a manner reversible by gibberellic acid. The results are consistent with a role for thioredoxin h in initiating the mobilization of nitrogen and carbon needed for germination and seedling development. Springer-Verlag, 1996 Abscisic acid nationallicence Gibberellic acid nationallicence Thioredoxin nationallicence Triticum (germination) nationallicence ABA : cis-abscisic acid nationallicence DTT : dithiothreitol nationallicence GA : gibberellin nationallicence GA3 : gibberellic acid nationallicence mBBr : monobromobimane nationallicence NTR : NADP-thioredoxin reductase nationallicence Lozano Rosa Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Wong Joshua Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Yee Boihon Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Peters Anne Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Kobrehel Karoly Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Buchanan Bob Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut Planta Springer-Verlag 200/1(1996-09-01), 100-106 0032-0935 200:1<100 1996 200 425 https://doi.org/10.1007/BF00196655 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196655 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lozano Rosa Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Wong Joshua Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Yee Boihon Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Peters Anne Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Kobrehel Karoly Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Buchanan Bob Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 100-106 0032-0935 200:1<100 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer