caa a22 4500 477099904 CHVBK 20180405111537.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196646 doi (NATIONALLICENCE)springer-10.1007/BF00196646 Changes in gene expression in the leaf of Lolium temulentum L. Ceres during the photoperiodic induction of flowering [Elektronische Daten] [Claire Périlleux, Philippe Ongena, Georges Bernier] Unifoliated plants of Lolium temulentum L. Ceres were induced to flower by a unique 24-h long day (LD) consisting of the extension of the regular 8-h short day (SD) (400 μmol photons·m−2·s−1, fluorescence + incandescence) with incandescence at 10-15 μmol photonsm −2·s−1. The polyadenylated-RNA complement of leaf blade tissues was analysed at 4-h intervals during the photoperiod extension in LD vs. SD, by using two-dimensional polyacrylamide gel electrophoresis to resolve in-vitro-translated products. Of the 991 spots that were analysed, none appeared or disappeared during the inductive cycle, i.e. no qualitative effect of floral induction was detected, at any time. Sixty-eight spots were found whose intensity was influenced by lengthening of the photoperiod; 50 of them, i.e. ca. 5% of the population analysed, were affected before the end of the extension period and were thus potentially related to floral induction. Many of these RNAs were not quantitatively constant during a 24-h cycle in SD. Seven of them oscillated according to the ‘light-on' and the ‘light-off' signals, among which three seemed to be controlled by phytochrome since their relative amount increased under the standard light conditions but decreased under incandescence even faster than in darkness. The large majority of other RNAs varied with a timing that was not clearly driven by the alternation of light and darkness, indicating that genes related to the biological clock may be especially sensitive to the lengthening of the photoperiod. Furthermore, seven spots were observed that underwent a phase-shift in LD, which consisted, for six of them, of a phase advance of 4-8 h. The steady-state level of CAB mRNA was analysed because the CAB gene family (encoding the chlorophyll a/b-binding proteins of the light-harvesting complexes) is known to be controlled both by the biological clock and phytochrome. In SD, the level was high in the light and low in darkness; the fluctuation was conducted by a circadian rhythm. When plants were exposed to the inductive LD, the peak of mRNA accumulation that was expected according to the endogenous rhythmicity was abolished, possibly because of the change in light quality during the LD extension. Springer-Verlag, 1996 Biological clock nationallicence CAB genes nationallicence Flowering(induction) nationallicence Gene expression nationallicence Lolium nationallicence Photoperiodism nationallicence Phytochrome nationallicence CAB : chlorophyll a/b-binding proteins of the light-harvesting complexes nationallicence 2D : two-dimensional nationallicence LD(s) : longday(s) nationallicence LDP(s) : long day plant(s) nationallicence SD(s) : short day(s) nationallicence SDP(s) : short day plant(s) nationallicence Périlleux Claire Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut Ongena Philippe Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut Bernier Georges Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut Planta Springer-Verlag 200/1(1996-09-01), 32-40 0032-0935 200:1<32 1996 200 425 https://doi.org/10.1007/BF00196646 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196646 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Périlleux Claire Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut NATIONALLICENCE 700 1- Ongena Philippe Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut NATIONALLICENCE 700 1- Bernier Georges Laboratoire de Physiologie végétale, Institut de Botanique, B22 Sart Tilman, B-4000, Liège, Belgium aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 32-40 0032-0935 200:1<32 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer