caa a22 4500 477099939 CHVBK 20180405111537.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196651 doi (NATIONALLICENCE)springer-10.1007/BF00196651 Developing tomato seeds when removed from the fruit produce multiple forms of germinative and post-germinative endo-β-mannanase. Responses to desiccation, abscisic acid and osmoticum [Elektronische Daten] [Birgit Voigt, J. Bewley] Several isoforms of endo-1,4β-D-mannanase (EC3.2.1.78) are produced in the endosperm and embryo of tomato (Lycopersicon esculentum Mill.) seed prior to the completion of germination. Other isoforms appear in the embryo and in the lateral endosperm following germination. This occurs in seeds removed from the fruit prior to completion of development at 45 d after pollination and placed directly on water, or following drying. Hence desiccation is not required to induce either germination- or post-germination-related mannanase activity. Incubating seeds in abscisic acid or osmoticum results in a reduction of both germination and total mannanase activity, but the isoforms that are produced in the embryo and micropylar region of the endosperm are identical to those produced in water-imbibed seeds prior to germination. Incubation of seeds in a high concentration of abscisic acid prevents all enzyme production. Only after the completion of germination does mannanase increase in the lateral regions of the endosperm. In contrast, mannanase is produced in the micropylar region regardless of whether the seed germinates or not. The isoforms produced in the two regions of the endosperm are different, those in the lateral endosperm being more similar to those produced in the cotyledons and axes of the embryo. Embryos and endosperms dissected prior to completion of germination and incubated separately produce far fewer isoforms than when these parts are together in the intact seed. Springer-Verlag, 1996 Abscisic acid nationallicence Desiccation nationallicence Endo-β-man-nanase nationallicence Endosperm nationallicence Lycopersicon (seed germination) nationallicence Osmoticum nationallicence ABA : cis-abscisic acid nationallicence DAP : days after pollination nationallicence GA : gibberellin nationallicence IEF : isoelectric focusing nationallicence PEG : polyethyleneglycol nationallicence pI : isoelectric point nationallicence Voigt Birgit Department of Botany, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut Bewley J. Department of Botany, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut Planta Springer-Verlag 200/1(1996-09-01), 71-77 0032-0935 200:1<71 1996 200 425 https://doi.org/10.1007/BF00196651 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196651 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Voigt Birgit Department of Botany, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Bewley J. Department of Botany, University of Guelph, NIG 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 71-77 0032-0935 200:1<71 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer