caa a22 4500 477099955 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196643 doi (NATIONALLICENCE)springer-10.1007/BF00196643 Immunocytochemical localization of phenylalanine ammonia-lyase in tissues of Populus kitakamiensis [Elektronische Daten] [Yuriko Osakabe, Kazuya Nanto, Hiroko Kitamura, Shinya Kawai, Yuki Kondo, Tomoyuki Fujii, Keiji Takabe, Yoshihiro Katayama, Noriyuki Morohoshi] The polypeptide encoded by the partial fragment of cDNA of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), PALcDNAl (Osakabe et al., 1995, Plant Sci. 105: 217-226), isolated from Populus kitakamiensis (P. sieboldii x P. grandidentata), was expressed in Escherichia coli cells. The polypeptide was purified and an antiserum raised against it. The antiserum recognized a protein of 77 kDa on nitrocellulose blots after sodium dodecyl sulfate-poly-acrylamide gel electrophoresis of total protein and the partially purified PAL protein from P. kitakamiensis. Moreover,the antiserum recognized a protein on the blot after non-denaturing polyacrylamide gel electrophoresis of P. kitakamiensis proteins and this protein had PAL activity. Furthermore, the antibody inhibited PAL activity of extracts from stem tissues. These results showed that the antiserum against the partial PAL peptide recognized only the PAL subunits in extracts of P. kitakamiensis. Immunolocalization studies of P. kitakamiensis tissues revealed that the PAL protein was specifically localized in the xylem and the phloem fibers and no immunogold signal was found in the epidermis, the cortex, the pith, or the cambium of either stems or leaves. Springer-Verlag, 1996 Phenylalanine ammonia-lyase nationallicence (PALcDNA1) nationallicence Populus nationallicence Lignin biosynthesis nationallicence IgG : immunoglobulin G nationallicence IPTG : isopropylthio-β-d-galactoside nationallicence PAL : phenylalanine ammonia-lyase nationallicence Osakabe Yuriko Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut Nanto Kazuya Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut Kitamura Hiroko Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut Kawai Shinya Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut Kondo Yuki Forestry and Forest Products Research Institute, 305, Tsukuba, Japan aut Fujii Tomoyuki Forestry and Forest Products Research Institute, 305, Tsukuba, Japan aut Takabe Keiji Faculty of Agriculture, Kyoto University, 606-01, Kyoto, Japan aut Katayama Yoshihiro Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Morohoshi Noriyuki Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Planta Springer-Verlag 200/1(1996-09-01), 13-19 0032-0935 200:1<13 1996 200 425 https://doi.org/10.1007/BF00196643 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196643 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osakabe Yuriko Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut NATIONALLICENCE 700 1- Nanto Kazuya Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut NATIONALLICENCE 700 1- Kitamura Hiroko Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawai Shinya Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183, Tokyo, Japan aut NATIONALLICENCE 700 1- Kondo Yuki Forestry and Forest Products Research Institute, 305, Tsukuba, Japan aut NATIONALLICENCE 700 1- Fujii Tomoyuki Forestry and Forest Products Research Institute, 305, Tsukuba, Japan aut NATIONALLICENCE 700 1- Takabe Keiji Faculty of Agriculture, Kyoto University, 606-01, Kyoto, Japan aut NATIONALLICENCE 700 1- Katayama Yoshihiro Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 700 1- Morohoshi Noriyuki Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 13-19 0032-0935 200:1<13 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer