caa a22 4500 47709998X CHVBK 20180405111538.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196661 doi (NATIONALLICENCE)springer-10.1007/BF00196661 An abscisic acid analog inhibits abscisic acid-induced freezing tolerance and protein accumulation, but not abscisic acid-induced sucrose uptake in a bromegrass ( Bromus inermis Leyss) cell culture [Elektronische Daten] [Ronald Wilen, Ping Fu, Albert Robertson, Suzanne Abrams, Nicholas Low, Lawrence Gusta] The application of abscisic acid (ABA), either as a racemic mixture or as optically resolved isomers, increases freezing tolerance in a bromegrass (Bromus inermis Leyss) cell culture and induces the accumulation of several heat-stable proteins. Two stereoisomers of an ABA analog, 2′3′ dihydroacetylenic abscisyl alcohol (DHA), were used to study the role of ABA-induced processes in the acquisition of freezing tolerance in these cells. Freezing tolerance was unchanged in the presence of (−) DHA (LT50 -9°C), and no increase in heat-stable protein accumulation was detected; however, the (+) enantiomer increased the freezing tolerance (LT50 -13°C) and induced the accumulation of these polypeptides. All three forms of ABA increased freezing tolerance in the bromegrass cells, although (−) ABA was less effective than either (+) or (±) ABA when added at equal concentrations. Cells pretreated with 20 or 50 μM (−) DHA displayed lower levels of freezing tolerance following the addition of 2.5, 7.5 or 25 μM (±) ABA. Full freezing tolerance could be restored by increasing the concentration of (±) ABA to > 25 μM. Pretreatment of cells with (−) DHA (20 or 50 μM) had no effect on freezing tolerance when 25 μM (+) ABA was added. The induction of freezing tolerance by 25 μM (−) ABA was completely inhibited by the presence of 20 μM (−) DHA. The accumulation of ABA-responsive heat-stable proteins was inhibited by pretreatment with 20 μM (−) DHA in cells treated with 2.5 or 7.5μM (+) ABA, and in cells treated with 25 μM (−) ABA. The accumulation of these polypeptides was restored when (±) or (+) ABA was added at a concentration of 25 μM. The analysis of proteins which cross-reacted with a dehydrin antibody revealed a similar inhibitory pattern as seen with the other ABA-responsive proteins. The effects of the various isomers of ABA and DHA on cell osmolarity and sucrose uptake was also investigated. In both cases, (±) and (+) ABA had pronounced effects on the parameters measured, whereas (−) ABA treated cells gave substantially different results. In both sucrose uptake and cell osmolarity, DHA had no significant effect on the results obtained following (±) or (+) ABA treatment. Maximum freezing tolerance was only observed in cells when both heat-stable protein accumulation and sucrose uptake were observed. Springer-Verlag, 1996 Abscisic acid nationallicence Bromus nationallicence Dehydrin nationallicence Freezing tolerance nationallicence Heat-stable protein nationallicence Sucrose nationallicence ABA : abscisic acid nationallicence DHA : 2′,3′ dihydroacetylenicabscisyl alcohols nationallicence DMSO : dimethyl sulfoxide nationallicence LT50 : temperature at which 50% of cells are killed nationallicence Wilen Ronald Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut Fu Ping Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut Robertson Albert Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut Abrams Suzanne Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Road, S7N OW9, Saskatoon, SK, Canada aut Low Nicholas Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut Gusta Lawrence Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut Planta Springer-Verlag 200/1(1996-09-01), 138-143 0032-0935 200:1<138 1996 200 425 https://doi.org/10.1007/BF00196661 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196661 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilen Ronald Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Fu Ping Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Robertson Albert Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Abrams Suzanne Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Road, S7N OW9, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Low Nicholas Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Gusta Lawrence Crop Development Centre, University of Saskatchewan, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 138-143 0032-0935 200:1<138 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer