caa a22 4500 477100058 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00196652 doi (NATIONALLICENCE)springer-10.1007/BF00196652 Molecular cloning of a P-type Ca2+-ATPase from the halotolerant alga Dunaliella bioculata [Elektronische Daten] [Birgit Raschke, Andreas Wolf] We have determined the complete primary structure of a putative P-type Ca2+ -ATPase from the unicellular, halotolerant alga Dunaliella bioculata. The protein (DBCA1) with a calculated molecular mass of 114 kDa and eight or ten putative transmembrane segments contains all amino acid motifs specific to the family of P-type ATPases. Highest homology scores were obtained by comparison with (sarco)endoplasmic reticulum-type plant and animal Ca2+-ATPases (54% identity, 70% similarity). In addition, all amino acids shown to be essential for Ca2+ transport in animal sarcoplasmic reticulum Ca2+-ATPases are preserved in DBCA1. Significantly lower homologies were found with animal plasma membrane Ca2+-ATPases (33% identity, 55% similarity), and the carboxyterminus of DBCA1 gave no indications of possible calmodulin-binding sites, characteristic of those enzymes. It is assumed that DBCA1 is a representative of the endomembrane class of Ca2+ ATPases. In Northern blot experiments with polyadenylated RNA, a 3.7-kb transcript was detected at levels which were very low compared with those of the plasma membrane H+-ATPase from D. bioculata (DBPMA1; Wolf et al., 1995, Plant Mol Biol 28: 657-666). Southern blot analyses suggest the existence of additional Ca2+-ATPase genes in the haploid genome of D. bioculata. Springer-Verlag, 1996 Ca2+ -ATPase nationallicence Calcium-homeostasis nationallicence Dunaliella nationallicence Endoplasmic reticulum nationallicence P-type ATPase nationallicence CaM : calmodulin nationallicence PM : plasma membrane nationallicence SER : (sarco)endoplasmic reticulum nationallicence Raschke Birgit Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-37073, Göttingen, Germany aut Wolf Andreas Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-37073, Göttingen, Germany aut Planta Springer-Verlag 200/1(1996-09-01), 78-84 0032-0935 200:1<78 1996 200 425 https://doi.org/10.1007/BF00196652 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196652 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raschke Birgit Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Wolf Andreas Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/1(1996-09-01), 78-84 0032-0935 200:1<78 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer