caa a22 4500 477100112 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00200304 doi (NATIONALLICENCE)springer-10.1007/BF00200304 The CO2 permeability of the plasma membrane of Chlamydomonas reinhardtii [Elektronische Daten] mass-spectrometric 18O-exchange measurements from 13C18O2 in suspensions of carbonic anhydrase-loaded plasma-membrane vesicles [Dieter Sültemeyer, Karl-Albert Rinast] The unicellular green alga Chlamydomonas reinhardtii possesses a CO2-concentrating mechanism. In order to measure the CO2 permeability coefficients of the plasma membranes (PMs), carbonic anhydrase (CA) loaded vesicles were isolated from C. reinhardtii grown either in air enriched with 50 mL CO2 · L−1} (high-Ci cells) or in ambient air (350 μL CO2 · L−1}; low-Ci cells). Marker-enzyme measurements indicated less than 1% contamination with thylakoid and mitochondrial membranes, and that more than 90% of the PMs from high and low-Ci cells were orientated right-side-out. The PMs appeared to be sealed as judged from the ability of vesicles to accumulate [14C]acetate along a proton gradient for at least 10 min. Carbonic anhydrase-loaded PMs from high and low-Ci cells of C. reinhardtii were used to measure the exchange of 18O between doubly labelled CO2 (13C18O2) and H2O in stirred suspensions by mass spectrometry. Analysis of the kinetics of the 18O depletion from 13C18O2 in the external medium provides a powerful tool to study CO2 diffusion across the PM to the active site of CA which catalyses 18O exchange only inside the vesicles but not in the external medium (Silverman et al., 1976, J Biol Chem 251: 4428-4435). The activity of CA within loaded PM vesicles was sufficient to speed-up the 18O loss to H2O to 45360-128800 times the uncatalysed rate, depending on the efficiency of CA-loading and PM isolation. From the 18O-depletion kinetics performed at pH 7.3 and 7.8, CO2 permeability coefficients of 0.76 and 1.49·10−3} cm·s−1}, respectively, were calculated for high Ci cells. The corresponding values for low-Ci cells were 1.21 and 1.8·10−3} cm·s−1}. The implications of the similar and rather high CO2 permeability coefficients (low CO2 resistance) in high and low-Ci cells for the COi-concentrating mechanism of C. reinhardtii are discussed. Springer-Verlag, 1996 Carbon dioxide-concentrating mechanism nationallicence Carbonic anhydrase nationallicence Chlamydomonas nationallicence Inorganic carbon nationallicence Plasma membrane (CO2 permeability) nationallicence AZA : acetazolamide nationallicence CA : carbonic anhydrase nationallicence CCCP : carbonylcyanide m-chlorophenylhydrazone nationallicence CCM : CO2concentrating mechanism nationallicence Ci : inorganic carbon nationallicence IDPase : inosine diphosphate nationallicence P : permeability coefficient nationallicence PM : plasma membrane nationallicence Rubisco : ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence Sültemeyer Dieter Fachbereich Biologie, Universität Kaiserslautern, Postfach 3049, D-67653, Kaiserslautern, Germany aut Rinast Karl-Albert Institut für Botanik der Technischen Hochschule, Schnittspahnstrasse 3-5, D-64387, Darmstadt, Germany aut Planta Springer-Verlag 200/3(1996-11-01), 358-368 0032-0935 200:3<358 1996 200 425 https://doi.org/10.1007/BF00200304 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00200304 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sültemeyer Dieter Fachbereich Biologie, Universität Kaiserslautern, Postfach 3049, D-67653, Kaiserslautern, Germany aut NATIONALLICENCE 700 1- Rinast Karl-Albert Institut für Botanik der Technischen Hochschule, Schnittspahnstrasse 3-5, D-64387, Darmstadt, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/3(1996-11-01), 358-368 0032-0935 200:3<358 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer