caa a22 4500 477100139 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00200302 doi (NATIONALLICENCE)springer-10.1007/BF00200302 Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization [Elektronische Daten] [J.-L. Wang, L. Walling, G. Jauh, Y.-Q. Gu, E. Lord] A cofactor-independent phosphoglycerate mutase (PGAM-i) was isolated to homogeneity from monocotyledonous Lilium longiflorum Thunb. Two-dimensional (2D) polyacrylamide gel electrophoresis resolved three PGAM-i forms. This enzyme was originally identified by cross-reactivity to antibodies affinity-purified from 2D gels using human vitronectin (VN). Antibody produced against a denatured protein spot from a 2D gel did not recognize VN protein, but partial protein and DNA sequencing showed similarity of the former protein to maize PGAM-i. Immunoblots from roots, styles, leaves, and anthers showed the presence of PGAM-i in all tissues examined; it was isolated predominantly from the soluble cell fraction, with some present in the insoluble cell fraction. Immunoelectron microscopy demonstrated its localization in the cytoplasm and plastids in root cells near the apical meristem. In addition, immunogold labeling detected signals from the nucleus. The immunohistochemical localization of the enzyme in the nucleus, as well as in the cytosol and plastids, indicates that lily PGAM-i might have multiple functions in the cell. Springer-Verlag, 1996 Antibody production nationallicence Enzyme immunolocalization nationallicence Lilium nationallicence Phosphoglycerate mutase (cofactor independent) nationallicence Vitronectin antibody nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence PGAM : cofactor-dependent phosphoglycerate mutase nationallicence PGAM-i : cofactor independent phosphoglycerate mutase nationallicence TEM : transmission electron microscopy nationallicence 2D : two dimensional nationallicence VN : vitronectin nationallicence Wang J.-L Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Walling L. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Jauh G. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Gu Y.-Q Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Lord E. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Planta Springer-Verlag 200/3(1996-11-01), 343-352 0032-0935 200:3<343 1996 200 425 https://doi.org/10.1007/BF00200302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00200302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang J.-L Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Walling L. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Jauh G. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Gu Y.-Q Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Lord E. Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/3(1996-11-01), 343-352 0032-0935 200:3<343 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer